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CD16 MicroBeads
Description
CD16 MicroBeads have been developed for the separation of NK cells from rhesus monkey PBMCs. CD16 is expressed on the majority of rhesus monkey NK cells1 and on a subset of monocytes.
Applications
Non-human primate CD16 MicroBeads can be used for positive selection of NK cells after monocyte depletion with CD56 MicroBeads (CD16+CD56- NK cell subset) or CD14 MicroBeads. CD16+ NK cells can, for example, be used for studies on NK cell cytotoxicity or signal transduction.
CD16 MicroBeads are suitable for depletion of NK cells, e.g., prior to positive selection of cytotoxic T cells using the CD8 MicroBead Kit.
Columns
For positive selection: MS, LS, XS, or autoMACS™ Columns. For depletion: LD, CS, D, or autoMACS Columns.
 
Figure 1
Positive selection of CD16+ NK cells from rhesus monkey PBMCs using CD16 MicroBeads, a MiniMACS™ Separator, and an MS Column. As some rhesus monkey monocytes express CD16, monocytes1 as well as a minor subset of CD56+ NK cells2 were depleted by using CD56 MicroBeads prior to positive selection of CD16+ cells.
PBMCs after depletion of CD56+ cells
CD16+ NK cells
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Products
CD16 MicroBeads, non-human primate
for 109 total cells
Download data sheet
130-091-145
Qty:
 

Related products
CD56 MicroBeads, non-human primate (#130-091-094)
CD14 MicroBeads, non-human primate (#130-091-097)
CD8 MicroBead Kit, non-human primate (#130-091-112)
CD8 Antibodies
CD16 Antibodies
CD56-PE, human (#130-090-755)
CD56-APC, human (#130-090-843)
MACS References
1. Carter et al. (1999) Cytometry 37: 41-50.[8299]
2. Webster and Johnson (2005) Immunology 115: 206-214.[8298]
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