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MACS® Cell Separation—the principle
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> The principle
> Labeling strategies
> Separation strategies
A poster depicting MACS Cell Separation Strategies can be downloaded here.
The poster is also available in print format, which additionally presents a variety of amazing images of cells separated with MACS Technology - please order your personal copy here.
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MACS Cell Separation
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MACS® Technology has become the standard method for cell separation. Numerous publications have proven its versatility for multiple applications: cell separations with consistent high quality results from lab bench to clinical applications, from small scale to large scale separations, from frequently occurring cells to rare cells and sophisticated subsets. More than 250 MACS Cell Separation Reagents are available for the efficient separation of virtually any cell type.
MACS Cell Separation is based on MACS MicroBeads, specific monoclonal antibodies conjugated to superparamagnetic particles, which are only 50 nanometers in size. Cells labeled with MACS MicroBeads are retained in the magnetic field within a MACS Column placed in a MACS Separator, allowing fast and efficient separation. Separated cells—the positively labeled fraction as well as the non-labeled fraction—can directly be used for downstream applications, such as cell analysis, further expansion, or functional assays. MACS MicroBeads are extremely small and biodegradable and the whole separation procedure is fast and gentle. Thus, the separated cells remain viable and their functionality is not impaired.
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- Excellent purity, recovery, and viability of separated cells
- Efficient separation of frequently occurring cells as well as rare cells
- Flexible separation strategies: positive selection, untouched isolation, or sequential sorting
- From small-scale to large-scale cell separation
- Manual or automated separation
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MACS MicroBeads
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- Small (50 nm) superparamagnetic particles coupled to highly specific antibodies
- Short incubation times (15 minutes)
- Non-toxic and biodegradable
- No bead detachment required: Labeled cells can go straight to flow cytometric analysis, cell culture, or other downstream applications
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MACS Columns and Separators
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- Gentle to cells
- Thorough rinsing procedure
- Convenient for working under sterile conditions
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