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| Embryonic stem cells (ESCs) and induced pluripotent stem (iPS) cells |
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Mouse ES and iPS cell research
To investigate embryonic stem (ES) and induced pluripotent stem (iPS) cells it is crucial to use homogenous cell populations. MACS® Technology enables the isolation and analysis of numerous particular cell types and subsets at high purity.
Separation of ES and iPS cells
MACS Technology allows the separation of undifferentiated cells from differentiated or feeder cells due to differential surface marker expression.
SSEA-1 is one of the hallmark mouse ES cell markers associated with a state of pluripotency and is known to be expressed on undifferentiated ES and iPS cells. Anti-SSEA-1 (CD15) MicroBeads can be used to separate mouse ES or iPS cells from feeder cells or to deplete unwanted pluripotent cells from differentiation cultures.
SSEA-1 is one of the first pluripotency markers expressed during the reprogramming of mouse cells to iPS cells. The number of iPS cells generated during reprogramming can be enhanced by enrichment of SSEA-1+ cells using Anti-SSEA-1 (CD15) MicroBeads.
Our scientists have identified a cell surface marker that is strongly upregulated during the early differentiation of mouse ES or iPS cells in culture. This discovery was used to establish a magnetic cell sorting protocol which allows easy depletion of the differentiated cells and results in homogeneous pluripotent stem cell cultures. The new Pluripotent Stem Cell Isolation Kit, mouse is the product of these developments and enables the rapid and gentle isolation of pluripotent stem cells.
Separation of ES- or iPS cell–derived progenitor cells
These cells express the same surface markers as their respective progenitors from adult tissue. MACS® Technology can be used to deplete unwanted cells during the differentiation process or to enrich the cells of interest by positive selection.
Various ES cell-derived cell types have been isolated by MACS Technology.
• HSCs have been separated with the CD117 MicroBeads.
• Endothelial cells were isolated with high purity using the Anti-Sca-1 MicroBead Kit (FITC).
• Smooth muscle cells were isolated according to the expression of Sca-1.
In general, ES- or iPS cell–derived hematopoietic and nonhematopoietic progenitors as well as neural stem and progenitor cells can be separated by using the same products as for adult stem cells or with MicroBeads for indirect magnetic labeling.
MACSmolecular products and services for analysis of ES and iPS cells |
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