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| Embryonic stem cells (ESCs) and induced pluripotent stem (iPS) cells |
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Mouse ESC and iPS cell research
Embryonic stem cells (ESCs) and induced pluripotent stem (iPS) cells have the capacity to self-renew and to differentiate into all cell types. They promise an essentially unlimited supply of specific cell types for basic research, drug testing, and in vivo studies.
To investigate these cells it is crucial to use homogenous cell populations. MACS® Technology enables the isolation and analysis of numerous particular cell types and subsets at high purity.
Separation of ESCs and iPS cells
MACS Technology allows the separation of undifferentiated cells from differentiated cells or feeder cells due to differential surface marker expression. For this purpose MicroBeads for indirect magnetic labeling can be used with virtually any primary antibody, e.g., SSEA-1 (CD15).
Prominin-1 expression is restricted to peripheral regions of ESC colonies and seems to be expressed by ESCs that just started to differentiate. Therefore, the Prominin-1 MicroBeads can be used to obtain pluripotent cells by depleting the early differentiated cells.
The Annexin-V MicroBeads can be used to deplete apoptotic cells from ESC cultures.
The generation of iPS cells can be enhanced by enrichment of SSEA-1 (CD15)+ cells. During the reprogramming of mouse fibroblasts to iPS cells, SSEA-1 is one of the first pluripotency markers that is expressed.
Separation of ESC- or iPS cell–derived progenitor cells
These cells express the same surface markers as their respective progenitors from adult tissue. MACS® Technology can be used to deplete unwanted cells during the differentiation process or to enrich the cells of interest by positive selection.
Various ESC-derived cell types have been isolated by MACS Technology.
• HSCs have been separated with the CD117 MicroBeads.
• Endothelial cells were isolated with high purity using the Anti-Sca-1 MicroBead Kit (FITC).
• Smooth muscle cells were isolated according to the expression of Sca-1.
In general, ESC- or iPS cell–derived hematopoietic and nonhematopoietic progenitors as well as neural stem and progenitor cells can be separated by using the same products as for adult stem cells or with MicroBeads for indirect magnetic labeling.
MACSmolecular products and services for analysis of ESCs and iPS cells |
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