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| CD154 – the superior marker for detection and enrichment of activated antigen-specific T cells |
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CD154 is transiently up-regulated on CD4+ T cells shortly after their activation and can therefore be used as a more reliable marker for activated antigen-specific T cells than traditionally used markers, such as CD25 or CD69. Its use in combination with cytokine detection enables easy access to the analysis of TH cells of a defined specificity. With MACS® Technology, pure, viable T cells can be conveniently isolated for downstream analysis.
- CD154 – a more reliable and specific marker than CD69
- The entire stimulated CD4+ cell population can be retrieved or analyzed.
- Easy to combine with cytokine analysis
Separation of CD154+ activated antigen-specific T cells:
CD154 MicroBead Kit, human
CD154 Enrichment and Detection Kit, mouse
Analysis of CD154+ activated antigen-specific T cells:
CD154 antibodies, human (-PE, -APC, -Biotin)
CD154 antibodies, mouse (-PE, -APC, -Biotin)
CD154 Detection Cocktails, mouse (-PE, -APC)
Intracellular staining kits
CD154/IFN-γ/CD4 Detection Kit, human
CD154/Il-2/CD4 Detection Kit, human
CD154/Il-4/CD4 Detection Kit, human
CD154/IL-5/CD4 Detection Kit, human
CD154/TNF-α/CD4 Detection Kit, human
References:
Frentsch et al. (2005) Nat. Med. 11: 1118-1124.
Chattopadhyay et al. (2005) Nat. Med. 11: 1113-1117.
Kirchhoff et al. (2007) Eur. J. Immunol. 37: 2370-2377.
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