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Miltenyi Biotec Miltenyi Biotec MACS® Cell Separation Reagents | for human cells | Cytokine-producing cells
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navigationMACS® Cell Separation Reagents
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for human cellsfor human cellsfor human cells
Stem and progenitor cellsStem and progenitor cellsStem and progenitor cellsStem and progenitor cells
T cellsT cellsT cellsT cells
NK cellsNK cellsNK cellsNK cells
B CellsB CellsB CellsB Cells
MonocytesMonocytesMonocytesMonocytes
Dendritic cellsDendritic cellsDendritic cellsDendritic cells
Antigen-presenting cellsAntigen-presenting cellsAntigen-presenting cellsAntigen-presenting cells
Granulocytes and myeloid cellsGranulocytes and myeloid cellsGranulocytes and myeloid cellsGranulocytes and myeloid cells
LeukocytesLeukocytesLeukocytesLeukocytes
Erythroid cellsErythroid cellsErythroid cellsErythroid cells
Megakaryocytes and plateletsMegakaryocytes and plateletsMegakaryocytes and plateletsMegakaryocytes and platelets
Endothelial cellsEndothelial cellsEndothelial cellsEndothelial cells
Epithelial cellsEpithelial cellsEpithelial cellsEpithelial cells
FibroblastsFibroblastsFibroblastsFibroblasts
Neural cellsNeural cellsNeural cellsNeural cells
Cytokine-producing cellsCytokine-producing cellsCytokine-producing cellsCytokine-producing cells
Tumor cellsTumor cellsTumor cellsTumor cells
Fc receptor blockingFc receptor blockingFc receptor blockingFc receptor blocking
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for non-human primate cellsfor non-human primate cellsfor non-human primate cells
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for mouse cellsfor mouse cellsfor mouse cells
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for rat cellsfor rat cellsfor rat cells
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for indirect magnetic labelingfor indirect magnetic labelingfor indirect magnetic labeling
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for apoptotic and dead cellsfor apoptotic and dead cellsfor apoptotic and dead cells
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for isolation of mitochondriafor isolation of mitochondriafor isolation of mitochondria
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MACS® Cell Separation Columns
MACS® Separators
autoMACS® Cell Separation
MACS® Cell Separation Buffers
MACS® Accessories
MACS® Products for sample preparation
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MACS® Cytokine Secretion Assays
MACS® Cytokine Secretion Assays
MACS® Cytokine Secretion Assays are designed for highly sensitive detection as well as optional enrichment of viable cytokine-secreting cells.
This unique technology allows:
  • Detection of secreted cytokines on single-cell level
  • Simultaneous phenotyping of cytokine-secreting cells for more detailed information on subsets and activation status
  • Detection of coexpression of two different cytokines or simultaneous staining with peptide-MHC tetramers for functional analysis of the cells
  • Magnetic enrichment of viable cytokine-secreting cells for enhanced sensitivity of detection of one antigen-specific T cell per million cells or for downstream applications

For polyclonal activation of human T cells, Miltenyi Biotec offers CytoStim and the T Cell Activation/Expansion Kit. Antigen-specific stimulation can be achieved with the CMV pp65 - Recombinant Protein or the NEW PepTivator™ CMV pp65, a peptide pool for CMV pp65-specific T cell stimulation.
Details and background information
Cytokine Secretion Assays – reference list [PDF, 147 KB]
Cytokine analysis – product list [PDF, 184 KB]
Cytokine Analysis Flyer [PDF, 1 MB]
IFN-α Secretion Assay - Cell Enrichment and Detection Kit
ProductApplicationsorder no.
IFN-α Secretion Assay – Cell Enrichment and Detection Kit (PE), humanIsolation and detection of human IFN-α–secreting cells.130-092-605
IFN-γ Secretion Assay - Cell Enrichment and Detection Kit
ProductApplicationsorder no.
IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE), humanIsolation and detection of human IFN-γ-secreting cells130-054-201
Large Scale IFN-γ Secretion Assay – Enrichment Kit
ProductApplicationsorder no.
Large Scale IFN-γ Secretion Assay – Enrichment Kit, humanIsolation of viable antigen-specific, IFN-γ-secreting human cells.130-091-329
IL-2 Secretion Assay – Cell Enrichment and Detection Kit
ProductApplicationsorder no.
IL-2 Secretion Assay – Cell Enrichment and Detection Kit (PE), humanIsolation and detection of human IL-2-secreting cells130-090-488
IL-4 Secretion Assay - Cell Enrichment and Detection Kit
ProductApplicationsorder no.
IL-4 Secretion Assay – Cell Enrichment and Detection Kit (PE), humanIsolation and detection of human IL-4-secreting cells130-054-101
IL-5 Secretion Assay – Cell Enrichment and Detection Kit
ProductApplicationsorder no.
IL-5 Secretion Assay – Cell Enrichment and Detection Kit (PE), humanIsolation and detection of human IL-5-secreting cells130-091-622
IL-10 Secretion Assay - Cell Enrichment and Detection Kit
ProductApplicationsorder no.
IL-10 Secretion Assay - Cell Enrichment and Detection Kit (PE), humanIsolation and detection of human IL-10-secreting cells130-090-435
IL-12 Secretion Assay – Cell Enrichment and Detection Kit
ProductApplicationsorder no.
IL-12 Secretion Assay – Cell Enrichment and Detection Kit (PE), humanIsolation and detection of human IL-12-secreting cells130-092-122
IL-13 Secretion Assay – Cell Enrichment and Detection Kit
ProductApplicationsorder no.
IL-13 Secretion Assay – Cell Enrichment and Detection Kit (PE), humanIsolation and detection of human IL-13-secreting cells130-093-480
IL-17 Secretion Assay – Cell Enrichment and Detection Kit
ProductApplicationsorder no.
IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE), humanDetection and isolation of viable human IL-17–secreting leukocytes from PBMCs130-094-542
TNF-α Secretion Assay – Cell Enrichment and Detection Kit
ProductApplicationsorder no.
TNF-α Secretion Assay – Cell Enrichment and Detection Kit (PE), humanEnrichment and detection of human TNF-α-secreting cells130-091-269
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