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| MACS® Cytokine Secretion Assays |
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MACS® Cytokine Secretion Assays are designed for highly sensitive detection as well as optional enrichment of viable cytokine-secreting cells.
This unique technology allows:
- Detection of secreted cytokines on single-cell level
- Simultaneous phenotyping of cytokine-secreting cells for more detailed information on subsets and activation status
- Detection of coexpression of two different cytokines or simultaneous staining with peptide-MHC tetramers for functional analysis of the cells
- Magnetic enrichment of viable cytokine-secreting cells for enhanced sensitivity of detection of one antigen-specific T cell per million cells or for downstream applications
For polyclonal activation of human T cells, Miltenyi Biotec offers CytoStim and the T Cell Activation/Expansion Kit. Antigen-specific stimulation can be achieved with the CMV pp65 - Recombinant Protein or the PepTivator® peptide pools for antigen-specific stimulation of CD4+ and CD8+ T cells. |
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| IFN-α Secretion Assay - Cell Enrichment and Detection Kit |
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| IFN-γ Secretion Assay - Cell Enrichment and Detection Kit |
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| Large Scale IFN-γ Secretion Assay – Enrichment Kit |
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| IL-2 Secretion Assay – Cell Enrichment and Detection Kit |
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| IL-4 Secretion Assay - Cell Enrichment and Detection Kit |
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| IL-5 Secretion Assay – Cell Enrichment and Detection Kit |
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| IL-10 Secretion Assay - Cell Enrichment and Detection Kit |
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| IL-12 Secretion Assay – Cell Enrichment and Detection Kit |
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| IL-13 Secretion Assay – Cell Enrichment and Detection Kit |
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| IL-17 Secretion Assay – Cell Enrichment and Detection Kit |
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| TNF-α Secretion Assay – Cell Enrichment and Detection Kit |
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