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| MACS® Intracellular Cytokine Staining |
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| Intracellular fluorescent staining of cytokines is a widely used technique for monitoring immune responses. After stimulation of the cells in the presence of a secretion inhibitor, the cells are fixed and permeabilized to be stained intracellularly. With our fluorochrome-conjugated anti-cytokine antibodies the procedure can either be performed in suspension or by solid-phase intracellular staining in combination with MACS® Technology. This gentle staining procedure minimizes loss of cells, thus, it is especially useful when working with samples containing low numbers of cells. |
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| Antibodies |
| Product | Clone /
Isotype | Applications | Format | MACS Control |
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| Anti-IFN-γ | AN18.17.24
Rat IgG | Intracellular staining of IFN-γ-producing mouse cells. | PE | |
| APC | |
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| Anti-IL-2 | JES6-5H4
Rat IgG2b | Intracellular staining of IL-2 producing mouse cells | PE | |
| APC | |
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| Anti-IL-17 | TC11-18H10
Rat IgG1 | Intracellular staining of IL-17 producing mouse cells | | |
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| Anti-TNF-α | MP6-XT22
Rat IgG1 | Intracellular staining of TNF-α producing mouse cells | FITC | |
| PE | |
| APC | |
| √ | Recommended for staining of magnetically separated cell fractions |
| (√) | Not recommended for cells that are labeled with MACS® MicroBeads using the same antigen |
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