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| MACS® Intracellular Cytokine Staining |
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| Intracellular fluorescent staining of cytokines is a widely used technique for monitoring immune responses. After stimulation of the cells in the presence of a secretion inhibitor, the cells are fixed and permeabilized to be stained intracellularly. With our fluorochrome-conjugated anti-cytokine antibodies the procedure can either be performed in suspension or by solid-phase intracellular staining in combination with MACS® Technology. This gentle staining procedure minimizes loss of cells, thus, it is especially useful when working with samples containing low numbers of cells. |
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| Antibodies |
| Product | Clone/
Isotype | Applications | Format | MACS
control |
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| Anti-IFN-α | LT27:295
Mouse IgG1 | Intracellular staining of IFN-α producing cells | FITC | |
| PE | |
| APC | |
| pure | |
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| Anti-IFN-γ | 45-15
Mouse IgG1 | Intracellular staining of human IFN-γ-producing cells | FITC | |
| PE | |
| APC | |
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| Anti-IL-2 | N7.48A
Mouse IgG2a | Intracellular staining of human IL-2-producing cells | PE | |
| APC | |
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| Anti-IL-4 | 7A3-3
Mouse IgG1 | Intracellular staining of human IL-4-producing cells | PE | |
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| Anti-IL-5 | JES1-39D10
Rat IgG2a | Intracellular staining of human IL-5-producing cells | PE | |
| APC | |
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| Anti-IL-10 | B-T10
Mouse IgG1 | Intracellular staining of human IL-10-producing cells | PE | |
| APC | |
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| Anti-IL-12 (p35/p70) | B-T21
Mouse IgG1 | Intracellular staining of IL-12–producing cells | PE | |
| APC | |
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| Anti-IL-12 (p40/p70) | 8.6
Mouse IgG1 | Intracellular staining of IL-12 producing cells | PE | |
| APC | |
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| Anti-IL-13 | JES10-5A2.2
Rat IgG1 | Intracellular staining of human IL-13-producing cells | PE | |
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| Anti-TNF-α | cA2
Human IgG1 | Intracellular staining of human TNF-α-producing cells | FITC | |
| PE | |
| APC | |
| √ | Recommended for staining of magnetically separated cell fractions |
| (√) | Not recommended for cells that are labeled with MACS® MicroBeads using the same antigen |
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