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| MACS® Intracellular Cytokine Detection Kits |
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Miltenyi Biotecs Intracellular Cytokine Detection Kits are designed for rapid and reliable flow cytometric detection of cytokine production in activated CD4+ T cells. As a specific and functional marker of T cell activation, CD154 is used. CD154 is transiently up-regulated on activated CD4+ T cells within hours after activation. By combining the detection of cytokine secreting cells with staining of CD154 and CD4, antigen specific TH cell responses can be directly identified.
The Kits contain all reagents necessary for the detection of cytokine-producing activated CD4+ T cells from whole blood or PBMCs by intracellular staining. In addition, reagents are included for the fixation and permeabilization of cells prior to immunofluorescent staining, as well as for the in vitro stimulation of T cells as a positive control. |
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| CD154/IFN-γ/CD4 Detection Kit |
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| CD154/IL-2/CD4 Detection Kit |
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| CD154/IL-4/CD4 Detection Kit |
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| CD154/IL-5/CD4 Detection Kit |
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| CD154/TNF-α/CD4 Detection Kit |
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