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MACS® Intracellular Cytokine Detection Kits
MACS<sup>®</sup> Intracellular Cytokine Detection Kits
Miltenyi Biotecs Intracellular Cytokine Detection Kits are designed for rapid and reliable flow cytometric detection of cytokine production in activated CD4+ T cells. As a specific and functional marker of T cell activation, CD154 is used. CD154 is transiently up-regulated on activated CD4+ T cells within hours after activation. By combining the detection of cytokine secreting cells with staining of CD154 and CD4, antigen specific TH cell responses can be directly identified.
The Kits contain all reagents necessary for the detection of cytokine-producing activated CD4+ T cells from whole blood or PBMCs by intracellular staining. In addition, reagents are included for the fixation and permeabilization of cells prior to immunofluorescent staining, as well as for the in vitro stimulation of T cells as a positive control.
Details and background information
Cytokine research – product list [PDF, 184 KB]
CD154/IFN-γ/CD4 Detection Kit
ProductApplicationsOrder no.
CD154/IFN-γ/CD4 Detection Kit, humanComplete kit for intracellular cytokine staining of activated, antigen-specific T cells130-092-814
CD154/IL-2/CD4 Detection Kit
ProductApplicationsOrder no.
CD154/IL-2/CD4 Detection Kit, humanComplete kit for intracellular cytokine staining of activated, antigen-specific T cells130-092-818
CD154/IL-4/CD4 Detection Kit
ProductApplicationsOrder no.
CD154/IL-4/CD4 Detection Kit, humanComplete kit for intracellular cytokine staining of activated, antigen-specific T cells130-092-817
CD154/IL-5/CD4 Detection Kit
ProductApplicationsOrder no.
CD154/IL-5/CD4 Detection Kit, humanComplete kit for intracellular cytokine staining of activated, antigen-specific T cells130-092-812
CD154/TNF-α/CD4 Detection Kit
ProductApplicationsOrder no.
CD154/TNF-α/CD4 Detection Kit, humanComplete kit for intracellular cytokine staining of activated, antigen-specific T cells130-092-813
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