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| gentleMACS™ Protocols for sample preparation |
| Preparation of single-cell suspensions from mouse spleen | PDF, 69 KB |
| Preparation of single-cell suspensions from mouse spleen with Collagenase D treatment | PDF, 73 KB |
| Preparation of single-cell suspensions from mouse lung with Collagenase D treatment | PDF, 74 KB |
| Preparation of single-cell suspensions from mouse liver with Collagenase IV treatment | PDF, 74 KB |
| Preparation of single-cell suspension from mouse heart with Collagenase II treatment | PDF, 82 KB |
| Preparation of single-cell suspensions from mouse neural tissue | PDF, 81 KB |
| Preparation of single-cell suspensions from implanted mouse tumors | PDF, 86 KB |
| Preparation of single-cell suspensions from human tumor tissues | PDF, 81 KB |
| Homogenization of tissue for total RNA isolation | PDF, 69 KB |
| Homogenization of tissue for mRNA isolation | PDF, 70 KB |
| Homogenization of tissue for protein extraction | PDF, 65 KB |
| Further protocols for sample preparation |
| Isolation of mononuclear cells from human peripheral blood by density gradient centrifugation | PDF, 89 KB |
| Isolation of mononuclear cells from human cord blood by density gradient centrifugation | PDF, 74 KB |
| Isolation of mononuclear cells from human bone marrow aspirates by density gradient centrifugation | PDF, 76 KB |
| Isolation of granulocytes from human peripheral blood by density gradient centrifugation | PDF, 83 KB |
| Preparation of cells from lymphoid tissue | PDF, 67 KB |
| Preparation of single-cell suspensions from mouse bone marrow | PDF, 58 KB |
| Preparation of the stromal vascular fraction (SVF) from human lipoaspirate | PDF, 117 KB |
| Isolation of human dermal microvascular endothelial cells (HDMECs) from foreskin tissue | PDF, 435 KB |
| Isolation of human umbilical vein endothelial cells (HUVECs) from umbilical cord | PDF, 195 KB |
| Dissection of retinas | PDF, 72 KB |
| Special protocols for cell separation |
| Isolation of CD34+HLA-DR- cells | PDF, 72 KB |
| Isolation of CD34+CD38- cells | PDF, 75 KB |
| Isolation of CD271 (LNGFR)+ MSCs/ADSCs from human lipoaspirate | PDF, 302 KB |
| Isolation of CD4+ T helper cells from human PBMCs | PDF, 63 KB |
| Isolation of CD4+CD294 (CRTH2)+ Th2 cells from human PBMCs | PDF, 265 KB |
| Isolation of CD4+CD62L+ T cells from human PBMCs | PDF, 303 KB |
| Isolation of CD8+CD62L+ T cells from human PBMCs | PDF, 345 KB |
| Enrichment of CD134 (OX40)+ cells | PDF, 265 KB |
| Isolation of CD10+ B cells from human bone marrow or lysed blood | PDF, 417 KB |
| Isolation of CD138 plasma cells from human PBMCs | PDF, 300 KB |
| Isolation of CD105+Sca-1+ LTR-HSCs from mouse bone marrow | PDF, 192 KB |
| Isolation of iNKT cells from mouse spleen using CD1d tetramers | PDF, 252 KB |
| Isolation of naive CD4+ T cells from non-human primate PBMCs | PDF, 214 KB |
| Isolation of CD4+CD25+CD127dim/– regulatory T cells from non-human primate PBMCs | PDF, 527 KB |
| Isolation of rat B lymphocytes | PDF, 99 KB |
| Schwann cell isolation from rat peripheral nerve tissue biopsies | PDF, 159 KB |
| Positive Selection of Antibody Expressing Hybridoma Cells | PDF, 77 KB |
| Special protocols for molecular applications |
| Chromatin immunoprecipitation (ChIP) | PDF, 94 KB |
| cDNA synthesis from whole blood using the μMACS cDNA Kit and subsequent KIR typing | PDF, 92 KB |
| Isolation of mRNA from stablized whole blood | PDF, 111 KB |
| Isolation of mRNA from yeast cells | PDF, 74 KB |
| Isolation of specific transcripts/RNAs | PDF, 107 KB |
| Isolation of specific tRNA molecules | PDF, 116 KB |
| Isolation of RNA-binding proteins | PDF, 128 KB |
| µMACS™ Streptavidin Kit—tips & hints | PDF, 91 KB |
| Biotinylation of plasmids | PDF, 47 KB |
| Subtractive hybridization | PDF, 88 KB |
| Phage display | PDF, 96 KB |
| Isolation of infectious HIV-1 | PDF, 106 KB |
| Isolation of infectious HIV using stimulated PBMCs | PDF, 162 KB |
| MACSelect Tag Vector cloning tips | PDF, 171 KB |
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