Download general, special and short protocols

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Below, you will find a collection of protocols which may be useful for your sample preparation and the realization of your specific experiment:

gentleMACS™ Protocols
Preparation of single-cell suspensions from mouse spleen	PDF, 55 KB
Preparation of single-cell suspensions from mouse spleen with Collagenase D treatment	PDF, 56 KB
Preparation of single-cell suspensions from mouse liver with Collagenase D treatment	PDF, 65 KB
Preparation of single-cell suspensions from mouse neural tissue	PDF, 115 KB
Homogenization of tissue for total RNA isolation	PDF, 72 KB
Homogenization of tissue for mRNA isolation	PDF, 72 KB

General protocols
Density gradient centrifugations	PDF, 104 KB
Preparation of cells from lymphoid tissue	PDF, 55 KB
Red blood cell lysis	PDF, 47 KB

Special cell separation protocols
Isolation of CD105+Sca-1+ LTR-HSCs from mouse bone marrow	PDF, 264 KB
Isolation of CD271 (LNGFR)⁺ MSCs/ADSCs from human lipoaspirate	PDF, 458 KB
Preparation of the stromal vascular fraction (SVF) from human lipoaspirate	PDF, 117 KB
Isolation of CD34⁺HLA-DR^- cells	PDF, 89 KB
Isolation of CD34⁺CD38^- cells	PDF, 89 KB
Isolation of CD4⁺ T Helper cells	PDF, 70 KB
Isolation of CD4⁺CD294 (CRTH2)⁺ Th2 cells	PDF, 161 KB
Isolation of CD4⁺CD45RA⁺ and CD4⁺CD45RA^- T helper cells from one sample	PDF, 96 KB
Isolation of CD4⁺CD62L⁺ T cells from human PBMCs	PDF, 312 KB
Isolation of CD8⁺CD62L⁺ T cells from human PBMCs	PDF, 348 KB
Isolation of CD19⁺CD27⁺ memory B cells from human PBMCs	PDF, 341 KB
Isolation of human surface IgG expressing memory B cells	PDF, 118 KB
Isolation of rat B lymphocytes	PDF, 148 KB
Positive Selection of Antibody Expressing Hybridoma Cells	PDF, 75 KB
Schwann cell isolation	PDF, 185 KB
Supplement to CD31 MicroBead Kit Booklet	PDF, 333 KB

Special and starting protocols for MACSmolecular
µMACS™ MACSelect Tag Vector cloning tips	PDF, 171 KB
µMACS™ mRNA isolation from stablized whole blood	PDF, 93 KB
HIV isolation using stimulated PBMCs	PDF, 162 KB
In-column DNAse I removal of genomic DNA	PDF, 131 KB
µMACS™ Streptavidin Kit—isolation of infectious HIV-1	PDF, 106 KB
µMACS™ Streptavidin Kit—isolation of specific transcripts	PDF, 58 KB
µMACS™ Streptavidin Kit—isolation of specific tRNA molecules	PDF, 55 KB
µMACS™ Streptavidin Kit—tips & hints	PDF, 91 KB
µMACS™ Streptavidin Kit—biotinylation of plasmids	PDF, 26 KB
µMACS™ mRNA isolation from yeast cells	PDF, 75 KB
µMACS™ Streptavidin Kit—phage display	PDF, 53 KB
µMACS™ Streptavidin Kit—RNA-binding protein isolation	PDF, 134 KB
µMACS™ Streptavidin Kit—serial analysis of gene expression (SAGE)	PDF, 150 KB
µMACS™ Streptavidin Kit—subtractive hybridization	PDF, 52 KB
MACSelect 4 and MACSelect K^K—transfection protocols	PDF, 140 KB

Short protocols for MACSmolecular
µMACS™ mRNA Isolation Kits – short protocol	PDF, 103 KB

Special protocols for Cytokine Secretion Assays
Expansion of antigen-specific cytokine-secreting T cells	PDF, 275 KB
Detection of cytokine secreting cells from whole blood	PDF, 71 KB
Detection and enrichment of cytokine secreting cells from whole blood	PDF, 142 KB
Two-color Cytokine Secretion Assays	PDF, 98 KB
Combined staining of cytokine-secreting cells with peptide-MHC tetramers	PDF, 102 KB

Short protocols for Cytokine Secretion Assays
Detection of cytokine secreting cells	PDF, 165 KB
Detection and enrichment of cytokine secreting cells	PDF, 202 KB

Special protocols for MACS® Media
NH ChondroDiff Medium - Differentiation of MSCs into chondrocytes and their detection	PDF, 192 KB

Special protocols for immunohistochemistry
Immunohistochemical detection of CD133 in paraffin-embedded sections	PDF, 658 KB

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