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Adult pluripotent stem cells
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The pluripotent differentiation potential of stem cells is defined by the ability to differentiate into cell types characteristic of any of the three developmental germ layers. Cells with this capacity have been found in fetal and adult cell sources.
Fetal-derived pluripotent stem cells from amniotic fluid cultures can be isolated according to the expression of CD1171 and share many adult and embryonic stem cell markers.
CD133+ cells isolated from mobilized peripheral blood partly adhere to plastic. These cells become negative for CD133 and are then capable of giving rise to non-adherent CD133+ cells. Non-adherent CD133+ cells were found in several organs after transplantation into NOD/SCID mice, whereas adherent cells showed the potential to differentiate into cell types of all three germ layers.2
For the investigation of the potential of different stem cell populations for possible clinical applications, Miltenyi Biotec has developed numerous tools.
Cell separation and analysis according to the expression of stem cell markers
Expansion and differentiation
microRNA and gene expression profiling and services
Stem cell–specific microRNA can be studied using miRXplore™ Microarray Kits and Services. Gene expression profiling services are offered with Agilent Whole Genome Microarrays.
Single-cell gene expression profiling SuperAmp™ Service*
for the amplification of RNA from only few to 10,000 cells sorted by flow cytometry, laser capture microdissection, primary cells, or tissue biopsies.
* In combination with the Microarray Service only.
For more information, see the flyer CD133 – the pluripotent stem cell marker.
References:
1. De Coppi et al. (2007) Nature Biotechnol. 25: 100–106.
2. Kuçi et al. (2008) Cell Prolif. 41: 12–27. |
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| Indirect CD133 MicroBead Kit |
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