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navigationMACS® Cell Separation Reagents
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for human cellsfor human cellsfor human cells
Stem and progenitor cellsStem and progenitor cellsStem and progenitor cellsStem and progenitor cells
ESCs and iPS cellsESCs and iPS cellsESCs and iPS cells>ESCs and iPS cells
Adult pluripotent stem cellsAdult pluripotent stem cellsAdult pluripotent stem cells>Adult pluripotent stem cells
Hematopoietic stem and progenitor cellsHematopoietic stem and progenitor cellsHematopoietic stem and progenitor cells>Hematopoietic stem and progenitor cells
Endothelial progenitor cellsEndothelial progenitor cellsEndothelial progenitor cells>Endothelial progenitor cells
Mesenchymal stromal cells (MSCs)Mesenchymal stromal cells (MSCs)Mesenchymal stromal cells (MSCs)>Mesenchymal stromal cells (MSCs)
Cancer stem cellsCancer stem cellsCancer stem cells>Cancer stem cells
T cellsT cellsT cellsT cells
NK cellsNK cellsNK cellsNK cells
B CellsB CellsB CellsB Cells
MonocytesMonocytesMonocytesMonocytes
Dendritic cellsDendritic cellsDendritic cellsDendritic cells
Antigen-presenting cellsAntigen-presenting cellsAntigen-presenting cellsAntigen-presenting cells
Granulocytes and myeloid cellsGranulocytes and myeloid cellsGranulocytes and myeloid cellsGranulocytes and myeloid cells
LeukocytesLeukocytesLeukocytesLeukocytes
Erythroid cellsErythroid cellsErythroid cellsErythroid cells
Megakaryocytes and plateletsMegakaryocytes and plateletsMegakaryocytes and plateletsMegakaryocytes and platelets
Endothelial cellsEndothelial cellsEndothelial cellsEndothelial cells
Epithelial cellsEpithelial cellsEpithelial cellsEpithelial cells
FibroblastsFibroblastsFibroblastsFibroblasts
Neural cellsNeural cellsNeural cellsNeural cells
Cytokine-producing cellsCytokine-producing cellsCytokine-producing cellsCytokine-producing cells
Tumor cellsTumor cellsTumor cellsTumor cells
Fc receptor blockingFc receptor blockingFc receptor blockingFc receptor blocking
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for non-human primate cellsfor non-human primate cellsfor non-human primate cells
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for mouse cellsfor mouse cellsfor mouse cells
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for rat cellsfor rat cellsfor rat cells
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for indirect magnetic labelingfor indirect magnetic labelingfor indirect magnetic labeling
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for apoptotic and dead cellsfor apoptotic and dead cellsfor apoptotic and dead cells
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for isolation of mitochondriafor isolation of mitochondriafor isolation of mitochondria
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MACS® Cell Separation Columns
MACS® Separators
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MACS® Cell Separation Buffers
MACS® Accessories
MACS® Products for sample preparation
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Adult pluripotent stem cells
<b>Adult pluripotent stem cells<br></b>
The pluripotent differentiation potential of stem cells is defined by the ability to differentiate into cell types characteristic of any of the three developmental germ layers. Cells with this capacity have been found in fetal and adult cell sources.

Fetal-derived pluripotent stem cells from amniotic fluid cultures can be isolated according to the expression of CD1171 and share many adult and embryonic stem cell markers.
CD133+ cells isolated from mobilized peripheral blood partly adhere to plastic. These cells become negative for CD133 and are then capable of giving rise to non-adherent CD133+ cells. Non-adherent CD133+ cells were found in several organs after transplantation into NOD/SCID mice, whereas adherent cells showed the potential to differentiate into cell types of all three germ layers.2

For the investigation of the potential of different stem cell populations for possible clinical applications, Miltenyi Biotec has developed numerous tools.

Cell separation and analysis according to the expression of stem cell markers

Expansion and differentiation

microRNA and gene expression profiling and services
Stem cell–specific miRNA and gene expression profiling can be performed using miRXplore™ Microarray Kits and Services

PIQOR™ Stem Cell Microarrays* and Services

Single-cell gene expression profiling SuperAmp™ Service**:
for the amplification of RNA from 1 to 100,000 cells sorted by flow cytometry, laser capture microdissection, primary cells, or tissue biopsies.


* PIQOR™ Microarrays Kits are not available in the US and Canada.
** In combination with the Microarray Service only.


For more information, see the flyer CD133 – the pluripotent stem cell marker.


References:
1. De Coppi et al. (2007) Nature Biotechnol. 25: 100–106.
2. Kuçi et al. (2008) Cell Prolif. 41: 12–27.
Details and background information
CD133 - the pluripotent stem cell marker [PDF, 586 KB]
Human HSC Flyer [PDF, 872 KB]
Human Stem Cell Flyer [PDF, 372 KB]
CD133 MicroBead Kit
ProductApplicationsorder no.
CD133 MicroBead Kit, humanPositive selection of human CD133+ stem and progenitor cells130-050-801
Indirect CD133 MicroBead Kit
ProductApplicationsorder no.
Indirect CD133 MicroBead Kit, humanPositive selection of human CD133+ stem and progenitor cells130-091-895
CD117 MicroBead Kit
ProductApplicationsorder no.
CD117 MicroBead Kit, humanPositive selection of human CD117 (c-kit)+ cells130-091-332
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