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| Isolation and analysis of epitope-tagged proteins |
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Epitope tagging is a powerful, versatile, and extremely popular technique for detecting and purifying proteins expressed by cloned genes.
The µMACS™ and MultiMACS™ Epitope Tag Isolation Kits are the first products that combines this attractive and simple method with all of the advantages of the MACS® Technology based on superparamagnetic beads.
The µMACS Epitope Tag Isolation Kits were developed for manual, low-throughput applications with the µMACS Separator.
The procedure can easily be upscaled with the MultiMACS Epitope Tag Isolation Kits to a semi- or fully-automated, high-throughput processing of up to 96 samples in parallel by utilizing the MultiMACS 96 Separator.
Both kit formats are available for the isolation of proteins tagged with:
- GFP (green fluorescent protein)
- HA (hemagglutinin)
- c-myc
- His (histidine-epitope)
- GST (glutathione-S-transferase)
The main advantages of MACS Technology for protein isolation are:
Sensitivity - fast reaction kinetics of small, non-sedimenting µMACS MicroBeads allow sensitive isolation even when working with rare proteins.
Specificity - high-quality monoclonal antibodies coupled to µMACS MicroBeads assure specific isolation of tagged proteins with low background.
Speed - less than 2 hours to high-purity proteins.
This product line is supplemented by a number of monoclonal Anti-Tag Antibodies suitable for analysis of epitope-tagged proteins by immunoblotting, ELISA, flow cytometry, or immunofluorescence microscopy. |
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| Isolation and analysis of GFP-tagged proteins |
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| Isolation and analysis of HA-tagged proteins |
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| Isolation and analysis of c-myc-tagged proteins |
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| Isolation and analysis of His-tagged proteins |
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| Isolation and analysis of GST-tagged proteins |
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