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| MACSelect™ System—smart enrichment of transfected cells in 3 hours |
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Many positives, low background
Using the magnetic MACSelect™ System on transfected primary cells or difficult-to-transfect cell lines leads to an up to 50-fold enrichment; thus, analysis of transfected cells is significantly more sensitive.
Time-saving
Transiently transfected cells are enriched in 2–3 hours instead of taking weeks to set up a stable cell line.
Gentle MACS® Technology
Biodegradable and non-toxic MACS® MicroBeads enable transfected cell selection without affecting cell function or viability. Truncated receptors as selection markers avoid an impact on cell function. Antibiotic treatment is no longer required.
No change of transfection method
The MACSelect System works with any transfection protocol: electroporation, calcium-phosphate, lipofection, or nucleofection. Just add the MACSelect Marker!
Smart
By coexpressing a marker on the cell surface, transfected cells can be magnetically labeled and separated from untransfected cells. Three different cell surface markers are available so that the MACSelect System is compatible with any cell type: human CD4, mouse MHC class I molecule H-2Kk, and human low-affinity nerve growth factor receptor (LNGFR). All pMACS vectors encode surface markers with truncated cytoplasmatic domains. Simply clone your gene-of-interest into a pMACS vector or use a pMACS cotransfection vector. For transfection, any method can be used. A few hours later, transfected cells are labeled with MACSelect MicroBeads and separated on a MACS Column by magnetic force. This can be achieved either manually using MS or LS Columns and a MACS Separator, or—for larger throughput—automated with the autoMACS Separator. MACSelect Antibodies facilitate the quality control of transfection and enrichment. |
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