Anti-MHC Class II (OX6) MicroBeads
Description Anti-MHC Class II (OX6) MicroBeads have been developed for the positive selection or depletion of rat cells expressing the major histocompatibility complex (MHC) class II. The monoclonal antibody recognizes a non-polymorphic determinant of the MHC class II antigen RT1B, the rat homolog of the murine I-A antigen. MHC class II (RT1B) is expressed on antigen-presenting cells (APCs), notably on B cells, dendritic cells, and on most monocytes and macrophages where it is up-regulated upon activation. After activation, epithelial cells, endothelial cells as well as microglia and NK cells also become MHC class II+. Applications Isolated MHC class II+ cells can be used for functional assays on antigen presentation and T cell stimulation. Additionally, Anti‑MHC Class II MicroBeads can be used for the enrichment of untouched T cells from lymph nodes by depletion of MHC class II+ cells. Columns For positive selection: MS, LS, XS, or autoMACS™ Columns. For depletion: LD, CS, D, or autoMACS Columns.		Figure 1 Isolation of T cells by depletion of MHC II (RT1B)+ cells from rat lymph nodes using Anti-MHC Class II (OX6) MicroBeads, a MiniMACS™ Separator, and an MS Column. A: Lymph node cells before separation B: Isolated T cells after depletion of OX6+ cells