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- Cutting-edge: Greater choice of fluorochromes
- Capable: Fast and sensitive rare cell analysis
- Accurate: Volumetric absolute cell counting
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The MACSQuant® VYB delivers all the performance, automation, compactness and convenience of the MACSQuant Analyzer but with a newly configured optical layout.
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Detect fluorescent proteins
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Optimal excitation of PE
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APC detection
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Minimal compensation
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Classic immunology
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Discover more
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Detect fluorescent proteins with ease
By incorporating a 561 nm laser and eight fluorescent channels, the MACSQuant VYB offers researchers a greater choice of fluorochromes to perform sophisticated multicolor experiments.
Analyze multiple fluorescent proteins simultaneously, such as mCherry, DsRed, GFP, CFP, and others. Benefit from a greater ability to detect red fluorescent proteins with four detection channels from the yellow laser.
Application note: Analysis of fluorescent proteins
Optimal excitation of PE with a 561nm laser
Profit from greater PE sensitivity for enhanced detection of cell populations showing only dim PE signals.
- Detect populations you may have missed with a blue laser.
- Take full advantage of improved PE tandem dyes, such as PE-Vio770™ or PE-Cy™7.
 Improved detection of PE fluorescence with the MACSQuant VYB. Cells expressing GFP under an Oct4 promoter and labeled with Anti-Feeder–PE, a dim fibroblast marker, were analyzed using a 488 nm blue laser on the MACSQuant Analyzer (lower panel) and a 561 nm yellow laser on the MACSQuant VYB (upper panel). All files displayed with no compensation applied. |
Four-laser functionality in a three-laser instrument
The MACSQuant VYB is designed for maximal versatility:
With a powerful 100 mW, 561 nm yellow laser, APC signals are clearly detected. APC tandem dyes, such as APC-Vio770™, APC-Cy™7, or APC-H7, are also easily detectable.
 Analysis of B cells stained with CD19-PE and CD20-APC using the MACSQuant VYB. |
Simplify analysis and compensation with spatially separated lasers
With FITC detection from the blue 488 nm laser and PE detection from the yellow 561 nm laser, there is little need for compensation between these dyes.
- Minimum compensation for the two most popular fluorochromes FITC (or GFP, Alexa488) and PE.
- Minimum compensation for 3-color assays with VioBlue®, FITC, and PE.
Minimal compensation between the two most popular dyes used in flow cytometry:
FITC and PE cells expressing GFP under an Oct4 promoter and labeled with Anti-Feeder-PE, a dim fibroblast marker, were analyzed using a 488 nm blue laser on the MACSQuant Analyzer (lower panel) and a 561 nm yellow laser on the MACSQuant VYB (upper panel). All files are displayed with no compensation applied. By analyzing both FITC and PE using the 488 nm blue laser with the MACSQuant Analyzer, major compensation is needed. However, when FITC and PE are analyzed with the MACSQuant VYB, minimal or no compensation is needed as FITC and PE are detected using the 488 nm blue laser and the 561 nm yellow laser, respectively. |
 Multicolor phenotyping: benefit from the MACSQuant VYB’s full potential
Perform classic immunophenotyping with the MACSQuant VYB. Shown here is a 6-color analysis of PBMCs with a panel of standard antibodies: CD45-VioBlue, CD3-FITC, CD4-PE, CD8-APC, CD45RA-APC-Cy7, and PI. |
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