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Direct magnetic labeling
Direct labeling with MACS® MicroBeads is the fastest way of magnetic labeling.
MACS MicroBeads specifically bind to antigens on the cell surface. Only one incubation step is required. Direct magnetic labeling requires a minimal number of washing steps and therefore minimizes cell loss.
Highly specific cell separation reagents for direct labeling of numerous cell types with MACS MicroBeads are available for human, mouse, rat, and non-human primate cells.
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Indirect magnetic labeling
Indirect magnetic labeling is based on a two-step procedure. In a first step, the cells are labeled with a primary antibody directed against a cell surface marker.
In a second step, the cells are magnetically labeled with MACS MicroBeads, which either bind to the primary antibody or to a molecule that is conjugated to the primary antibody.
The primary antibody can either be unconjugated, biotinylated, or fluorochrome-conjugated. Accordingly, magnetic labeling is achieved with Anti-Immunoglobulin MicroBeads, Anti-Biotin MicroBeads, or Anti-Fluorochrome MicroBeads.
Indirect labeling can also be performed by using a cocktail of primary antibodies to concurrently label a number of unwanted cell types, for example, for the untouched isolation of target cells.
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