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microRNA expression
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microRNA Expression Profiling Service – specificity down to a single nucleotide
Miltenyi Biotec offers two different Microarray platforms for reliable microRNA expression profiling studies.
If specificity counts
The miRXplore Microarray Platform was developed by Miltenyi Biotec in close collaboration with leading scientists (Landgraf & Tuschl et al.; 2007; Cell). This microarray platform sets the benchmark in terms of specificity in microarray-based microRNA profiling analysis. With the miRXplore Microarray Service it is even possible to discriminate between closely-related microRNAs with just a single nucleotide exchange.
The miRXplore Microarray Universal Reference Service is available for the comparison of multiple samples. The miRXplore Universal Reference (UR), a synthetic microRNA pool, is used for this purpose. Samples and controls are hybridized and compared to the miRXplore Universal Reference on individual microarrays. Subsequently, re-ratios are calculated to compare samples versus control, hence saving sample material.
The Bioinformatics Service Absolute Quantification miRXplore further utilizes the advantages of the miRXplore Universal Reference and enables a global, absolute quantification of microRNAs (Bissels & Tuschl et al.; 2009; RNA). The defined microRNA concentration in the miRXplore Universal Reference allows to determine the absolute microRNA concentration in your sample by translating the respective signal intensities.
If sensitivity counts
The Agilent microRNA Microarray Service only requires small input amounts of total RNA in the 100 nanogram range. This service uses a high-yield labeling method and does not require size fractionation or amplification steps that may introduce undesired bias during microRNA profiling. Download a product note provided by Agilent and learn more about their microRNA microarray platform.
Send us your samples as fresh or FFPE tissue and we’ll do the rest: microRNA extraction with our RNA Extraction Service or our FFPE Sample Preparation Service.
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