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Tissue
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Broad range of tissue sample preparation skills
The outcome of a microarray experiment depends on the quality of sample preparation. The experienced Genomic Service Team possesses a broad range of tissue sample preparation skills and uses well-established methods for different sample sources, ranging from cryoconserved tissues to tissue biopsies, from LCM (Laser Capture Microdissection) samples to particularly demanding FFPE (formalin-fixed-paraffin embedded) tissues.
The isolation of total RNA from cryoconserved tissues requires a fast and thorough homogenization of the starting material. With the gentleMACS™ Dissociator Miltenyi Biotec has developed a powerful tool that meets this requirement with optimized homogenization protocols.
The SuperAmp™ Service enables the performance of reliable microarray analysis from tissue sources with limited cell numbers, such as tissue biopsies and LCM samples from tissue sections. Based on their experience with LCM samples, the Genomic Services Team is aware of the pitfalls of sample collection and can provide qualified recommendations which are summarized in our guidelines for tissue sample preparation and submission.
Archived biopsies are a precious source of information on molecular causes of various diseases and the sequelae of disease at a molecular level. The Microarray Services portfolio includes preparation of the particularly challenging FFPE (formalin-fixed-paraffin embedded) tissue samples. Established methods are used to ensure successful microarray experiments for gene expression profiling and array-CGH analysis.
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