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Quality control
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Extensive control and documentation of sample quality
Successful and reliable microarray analysis depends on the quality of the utilized RNA and DNA samples. Furthermore, an exact quantification is necessary to determine the appropriate amount of starting material.
We apply state-of-the-art quality control (QC) tools and methods to meet these demands.
RNA samples are quantified using the NanoDrop spectrophotometer and quality-checked via the Agilent 2100 Bioanalyzer platform (Agilent Technologies). The results of the Bioanalyzer run are visualized in a gel image and an electropherogram using the Agilent 2100 Bioanalyzer expert software. In addition to this visual control, the software allows the generation of an RNA Integrity Number (RIN) to check integrity and overall quality of RNA samples.
Quantitation of genomic DNA samples is performed using the Qubit fluorometer and the Quant-iT dsDNA BR Assay Kit (Invitrogen), the integrity is assessed via agarose gel electrophoresis.
All QC technologies are well-established and have the advantages of high sensitivity and accuracy, and require minimal amounts of sample.
The QC results are then documented in an extensive QC Report. This report is sent via email to the customer with a recommendation regarding the applicability of the samples.
Our service is much more than simply assessing the quality and quantity of RNA and DNA samples. If necessary we can offer a variety of solutions to improve the RNA and DNA quality, such as DNAse treatment of RNA samples, proteinase treatment of DNA samples and re-purification and recommendations for improvement of sample collection.
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