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Blood
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Various approaches for blood samples
Microarray analysis from blood samples involves various approaches for this demanding sample source, which comprises fresh or stabilized whole blood samples, blood fractions (e.g. PBMCs, serum, plasma) and separated blood cells.
Choosing the right starting material is crucial to meaningful and reliable microarray results. The experienced Genomic Service Team can offer a variety of recommendations and solutions to meet this demand.
Non-stabilized whole blood samples (EDTA, citrate) are only suitable for array-CGH analysis. Their usage for gene expression profiling should be avoided since the samples have to be processed immediately and the expression profile may change during short storage.
Therefore, whole blood stabilization procedures, such as the standardized PAXgene™ Blood tubes (Qiagen) or Tempus™ Blood Tubes (Applied Biosystems), have to be used.
Miltenyi Biotec has developed numerous state-of-the-art tools for magnetic cell separation directly from whole blood Microarray analysis of whole blood samples involve the risk that differences in blood cell composition interfere with the expression profile. Gene expression analysis of isolated blood cells is highly recommended in order to circumvent this problem. Highly enriched and pure cell fractions can be obtained by using MACS Whole Blood Microbeads, tools for the gentle isolation of numerous leukocytes subsets. This reliable cell separation procedure can easily be standardized and used in an automated fashion with the autoMACS® Pro Separator. This synergy of MACS Technology and genomic analysis is called MACS-4-omics.
Even rare cell populations resulting from blood cell separation can be further analyzed by using the unique SuperAmp™ Service.
All information and recommendations are summarized in our guidelines for blood sample preparation and submission.
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