| Questions & answers |
| Which lasers are used in the MACSQuant® Analyzer optical bench? |
| Violet (405nm) Blue (488nm - diode pumped solid state) Red (635nm) |
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| What are the fluorescence sensitivities of the MACSQuant® Analyzer? |
| MESF: FITC <200; PE <100; APC <150 |
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| What is the error in absolute cell count? |
| The error in the absolute cell count is less than 5% CV |
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| What is the dynamic range of sensitivity? |
| The dynamic Range for FSC/SSC is 1 µm – 15 µm |
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| What type of sample tubes can I use? |
| The MACSQuant® Analyzer with the single tube holder or the MACS® MiniSampler can process many types of sample tubes. Microcentrifuge tubes, 5-, 10-, 15- mL tubes and 96-well microtiter plates can be automatically processed by the MACSQuant Analyzer. |
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| How many events can be acquired on the MACSQuant® Analyzer? |
| The MACSQuant can acquire up to 10,000 events per second |
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| At what speed can the MACSQuant® Analyzer analyze samples? |
| The MACSQuant Analyzer has three different flow rates Low (25uL/min), Med (50uL/min) and High (100 uL/min) |
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| What is the minimum sample volume that can be analyzed by the MACSQuant Analyzer? |
| 25 µL. |
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| What are the dimensions of the flow cell? |
| The flow cell is 200 × 250 µm and has a rectangular profile |
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| What is the laser spot for the MACSQuant® Analyzer? |
| The Laser Spot is 15 × 45 µm |
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| What is the carryover between samples? |
| The carryover between samples on the MACSQuant Analyzer is <0.01% |
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| What are the specifications of the workstation? |
The MACSQuant® Analyzer workstation includes:
PC with intel Pentium M 1.8 GHz
Memory 1 GB RAM
Data Storage: 40GB hard drive DVD RW drive
Networking: Ethernet
Monitor: 12 inch, touch screen
2x USB port Analog RGB / VGA port
COM Port (DSUB-9) |
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| What are the dimensions of the MACSQuant® Analyzer? |
| The Size (W × D × H) of the MACSQuant is 600 × 350 × 400 mm |
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| What are the optimal operating conditions? |
| The optimal operating temperature is between 15 to 25°C with humidity ranging from 10 to 90%. |
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| What is the power usage of the MACSQuant ®Analyzer? |
| The power is 350 W |
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| What type of sheath fluid should I use? |
| Miltenyi Biotec has specially designed buffers |
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| Does Miltenyi Biotec offer flow cytometry analysis software? |
| Yes, MACSQuantify™ Software is a user-friendly software that can be used with data acquired from the MACSQuant® Analyzer and other flow cytometers (fcs2.0 and fcs3.0 file formats) |
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| How many logarithmic decades does the MACSQuantify™ Software display? |
| The MACSQuantify Software displays 5-decade logarithmic scales |
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| What is the signal resolution? |
| The signal resolution of the MACSQuant® Analyzer is 16bit (65,536 channels) on all parameters |
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| Does the MACSQuant® Analyzer display data in bi-exponential or hyperlog scale? |
| The MACSQuantify™Software, which runs on the MACSQuant® Analyzer, utilizes a new version of scale called hlog, which is similar to bi-exponential and hyperlog scales. |
