NEW: Anti-iNKT MicroBeads for the isolation of human invariant natural killer T cells expressing the TCR Vα24-Jα18.

NEW: Enumeration of circulating endothelial progenitor cells (cEPCs) with the EPC Enrichment and Enumeration Kit, human, based on CD34, CD133, and CD309 (VEGFR-2/KDR) expression.

Thu, 18 Dec 2008 15:53:38 +0100

The EPC Enrichment and Enumeration Kit is designed for the enumeration of circulating EPCs (cEPCs) from peripheral blood, cord blood, or leukapheresis products as well as EPCs from bone marrow, following the pre-enrichment of EPCs.
Endothelial progenitor cells (EPCs) are defined by the expression of CD34, CD133, and CD309 (VEGFR-2/KDR).
The kit contains all reagents for red blood cell lysis, EPC enrichment, blocking of Fc Receptor–mediated non-specific labeling of non-target cells, enumeration of EPCs based on expression of CD34, CD133, and CD309 and exclusion of CD14+ cells, isotype controls, as well as for the exclusion of dead cells.

NEW: NK Cell Activation/Expansion Kit: the easy and efficient way to expand human natural killer cells

Fri, 05 Dec 2008 17:45:34 +0100

The NK Cell Activation/Expansion Kit is designed to activate and expand human NK cells. The kit consists of Anti-Biotin MACSiBead™ Particles and biotinylated antibodies against human CD335 (NKp46) and CD2. Anti-Biotin MACSiBead Particles loaded with biotinylated antibodies are used to activate and expand resting NK cells purified from human blood or PBMCs.

NEW: Kit for the isolation of CD4+ Recent Thymic Emigrants

Fri, 05 Dec 2008 17:42:47 +0100

The CD4+ Recent Thymic Emigrant Isolation Kit is designed for the isolation of CD4+CD45RA+CD31+ recent thymic emigrants (RTE) from human PBMCs.
The isolation is performed in a two-step procedure. First, non-CD4+ and memory CD4+ T cells are indirectly magnetically labeled with a cocktail of biotinylated antibodies and Anti-Biotin MicroBeads. The labeled cells are subsequently depleted over a MACS® Column. In the second step, CD4+CD45RA+CD31+ RTEs are directly labeled with CD31 MicroBeads and isolated by positive selection from the pre-enriched naive CD4+ T cell fraction.
RTEs are a subpopulation of naive T cells characterized by their content of T cell receptor excision circles (TRECs).
CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) has been described as a marker to identify the CD4+ RTEs among the naive CD4+ T cell population, as its expression correlates with the TREC content of CD4+ T cells. CD4+ T cells lacking CD31 expression harbor little or no TRECs, suggesting that they already proliferated in the periphery.
Therefore, the magnetic isolation of CD4+ RTEs is possible by direct isolation of CD31+ cells from pre-enriched, untouched naive CD4+ T cells.

NEW: Kit for the isolation of naive human CD4+ T cells

Tue, 02 Dec 2008 15:35:05 +0100

The Naive CD4+ T Cell Isolation Kit II provides a fast and efficient way to isolate untouched naive CD4+ T cells from human peripheral blood. It is the new generation of the Naive CD4+ T Cell Isolation Kit, for even higher purities of the isolated naive T helper cell population.
For magnetic depletion of memory CD4+ T cells and non-CD4+ T cells, PBMCs are incubated with a cocktail of biotinylated CD45RO, CD8, CD14, CD15, CD16, CD19, CD25, CD34, CD36, CD56, CD123, anti-TCRγ/δ, anti-HLA-DR, and CD235a (glycophorin A) antibodies. These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads for depletion.

NEW: Kit for the isolation of human CD4+CD25+CD45RA+ regulatory T cells

Tue, 02 Dec 2008 15:32:51 +0100

The CD4+CD25+CD45RA+ Regulatory T Cell Isolation Kit has been developed for the isolation of CD45RA+ regulatory T cells from human PBMCs. The isolation is performed in a two-step procedure. First, non-CD4+ and memory T cells are indirectly magnetically labeled with a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. The labeled cells are subsequently depleted by separation over a MACS® Column.
In the second step, CD4+CD25+CD45RA+ T cells are directly labeled with CD25 MicroBeads and isolated by positive selection from the pre-enriched CD4+CD45RA+ T cell fraction.
Regulatory CD4+CD25+ T cells isolated from the CD45RA+ naive T cell compartment were shown to be optimal for in vitro expansion. These expanded regulatory T cells maintained the FoxP3+ phenotype and their suppressive activity, whereas CD45RA– regulatory T cells from the memory T cell compartment lost FoxP3 expression and showed only modest suppressive activity.

STIMULATING: PepTivator™ Survivin 1 and PepTivator™ Melan-A/MART-1: peptide pools for in vitro stimulation of antigen-specific human T cells

Fri, 05 Dec 2008 16:52:36 +0100

PepTivator™ Peptide Pools consist of mainly 15-mer peptides with 11–amino acid (aa) overlap, covering the complete sequence of the respective antigen. They have been developed for efficient in vitro stimulation of antigen-specific CD4+ and CD8+ T cells.

NEW: Efficient activation and expansion of mouse T cells with the new T Cell Activation/Expansion Kit, mouse

Thu, 27 Nov 2008 17:18:20 +0100

The T Cell Activation/Expansion Kit is designed to activate and expand mouse T cells. The kit consists of Anti-Biotin MACSiBead™ Particles and biotinylated antibodies against mouse CD3ε and CD28. Anti-Biotin MACSiBead Particles loaded with biotinylated antibodies are used to mimic antigen-presenting cells and activate resting T cells in single-cell suspensions from spleen or lymph nodes as well as purified T cells. T cell expansion is achieved by culturing and reactivation at day 6–8 of culture.

NEW: MACS® Control Cocktails for separation control of human CD3+, CD4+, and CD8+ T cells

Thu, 27 Nov 2008 17:24:48 +0100

The MACS® Control (MC) Antibody Cocktails are a new product group, designed for the easy and rapid evaluation of cell separation efficiency and purity by flow cytometry. After separation with MACS Technology, cells are simply incubated with the pre-mixed cocktail and analyzed subsequently. The cocktail is optimized for separation control using the MACSQuant™ Analyzer, but can be used on other flow cytometers as well.
Due to the addition of a CD45 antibody conjugated to VioBlue™, the new fluorophore developed by Miltenyi Biotec for use with a violet laser, analysis can be easily restricted to leukocytes.
MC Control Cocktails are now available for CD3+, CD4+, and CD8+ human T cells.

NEW: gentleMACS™ Protocol for the dissociation of mouse lung tissue

Thu, 27 Nov 2008 17:22:51 +0100

A new protocol is now available for the preparation of single-cell suspensions from mouse lung tissue using the gentleMACS™ Dissociator. The tissue dissociation procedure is gentle to cells and yields cell suspensions with a high viability rate.

STIMULATING: PepTivator™ EBV BZLF1—peptide pool for efficient in vitro stimulation of human EBV-specific T cells

Tue, 04 Nov 2008 11:57:00 +0100

The PepTivator™ EBV BZLF1 is a peptide pool that consists mainly of 15-mer peptides with 11 amino acid (aa) overlap. It has been developed for efficient in vitro stimulation of EBV BZLF1–specific CD4+ and CD8+ T cells as peptides of 15-aa length with 11-aa overlap represent an optimized solution for stimulating both CD4+ and CD8+ T cells in various applications.
Epstein-Barr virus (EBV) is a human γ-herpesvirus with B cell growth–transforming ability and lymphomagenic potential. More than 90% of human adults are infected with EBV. Usually the primary infection is asymptomatic, but some individuals develop an infectious mononucleosis, a self-limiting lymphoproliferative disease.
BZLF1 activates a promoter of an EBV gene and plays a key role in the switch from latent to productive infection of EBV. BZLF1-specific CD4+ and CD8+ T cells have been found in EBV-infected individuals.

NEW: MACS Cell Isolation Kits for research on antigen-specific and tumor-specific human B cells: IgM+ Memory B Cell Isolation Kit, IgG+ Memory B Cell Isolation Kit, and B Cell Isolation Kit (B-CLL)

Tue, 04 Nov 2008 11:47:00 +0100

Miltenyi Biotec has significantly expanded the portfolio of products for B cell research. In particular, the isolation of memory B cell subsets and B cells of B-CLL or other B cell disease states from human PBMCs is greatly facilitated by new kits.

NEW: Products for the isolation, staining, and optimized culture of MSCA-1 (W8B2)–positive mesenchymal stromal cells

Tue, 04 Nov 2008 11:53:40 +0100

The Anti-MSCA-1 (W8B2) MicroBead Kit, based on the antibody clone W8B2, was developed for the isolation of mesenchymal stromal cells (MSCs) from bone marrow aspirates. The kit contains all necessary reagents for the magnetic isolation of MSCA-1⁺ cells, including Anti-MSCA-1 (W8B2) MicroBeads for magnetic labeling and FcR Blocking Reagent to block non-specific labeling via Fc receptors. The Anti-MSCA-1 (W8B2) MicroBeads recognize the mesenchymal stromal cell antigen-1 (MSCA-1), a so far unknown antigen. MSCA-1 was shown to be restricted to MSCs in the CD271^bright population in bone marrow. These CD271^brightCD45^dim MSCs have a much higher clonogenic capacity compared to the CD271⁺CD45⁺ fraction in bone marrow. Therefore, MSCA-1 is a highly specific marker to isolate MSCs with a high proliferative potential from bone marrow. MSCA-1 expression was not found on placenta-derived MSCs after culture. For the optimal separation and subsequent expansion of MSCA-1⁺ cells, the MSC Research Tool Box – MSCA-1 (W8B2) is available.
A variety of antibodies allow the identification and enumeration of MSCA-1⁺ cells by flow cytometry and fluorescence microscopy.

NEW: MultiMACS™ Epitope Tag Isolation Kits for fully-automated, 96-well isolation of c-myc-, GFP-, GST-, HA-, or His-tagged proteins

Tue, 05 Aug 2008 12:01:15 +0200

Epitope tagging is a powerful, versatile, and extremely popular technique for detecting and purifying proteins expressed by cloned genes.
The MultiMACS™ Epitope Tag Isolation Kits combine this attractive and simple method with all of the advantages of the MACS® Technology based on superparamagnetic beads.
MultiMACS Epitope Tag Isolation Kits allow the semi- or fully-automated, high-throughput processing of up to 96 samples in parallel by utilizing the MultiMACS 96 Separator.

NEW: EasyProbe Transcription Templates for an easy generation of high-quality, sequence-verified in situ probes

Tue, 05 Aug 2008 11:51:29 +0200

In situ hybridization (ISH) and northern blot analysis are common and powerful tools for the detection and quantification of mRNA. To achieve reliable and reproducible results, it is essential to use single-stranded RNA probes that have a high sequence specificity.
Often, the generation of these high-quality RNA probes turns out to be more tedious and time-consuming than expected.
Miltenyi Biotec's sequence-verified EasyProbe Transcription Templates are ready-to-use, linear DNA templates (in vitro transcription templates) for the generation of specific RNA probes for in situ hybridization or northern blot analysis.

NEW: Whole Blood Column Kit for the isolation of leukocyte subsets directly from human whole blood

Tue, 05 Aug 2008 12:14:39 +0200

Whole Blood Columns are designed for the separation of cells directly from whole blood. No density gradient centrifugation is required. Cells are magnetically labeled using Whole Blood MicroBeads. Separation is performed using a MidiMACS™, QuadroMACS™, VarioMACS™, or SuperMACS™ II Separator.
Whole Blood MicroBeads are available for the isolation of numerous leukocyte subsets.

NEW: VioBlue, the new dye for use with violet lasers expands your options for multicolor flow cytometry

Tue, 05 Aug 2008 11:42:38 +0200

Miltenyi Biotec is proud to introduce a new dye for use with a violet laser--VioBlue. With an absorption maximum at 405 nm and an emission maximum at 455 nm, it is perfectly suited for the standard laser and filter equipment of the MACSQuant™ Analyzer. VioBlue is also compatible with any other flow cytometer equipped with an appropriate laser. The multiplexing of VioBlue with other fluorochromes is easily possible, adding to the variety of marker combinations for multiparameter cell analysis.
Numerous MACS Antibodies conjugated to VioBlue are available and the product range is continuously growing.

Th17 CELLS: Products for the isolation and analysis of mouse Th17 cells

Thu, 31 Jul 2008 09:18:43 +0200

Th17 cells, a T helper cell subset distinct from Th1 and Th2 cells, preferentially produce interleukin-17 (IL-17, or IL-17A), IL-17F, IL-21, and IL-22. Th17 cells are crucial for the cross-talk between immune system and tissues and are responsible for driving immune inflammation.
Viable mouse IL-17–secreting cells can now be analyzed and isolated using the unique MACS® Cytokine Secretion Assays.

NEW: CD15 MACSiBead™ Kit, human, for quick depletion of granulocytes from whole blood

Thu, 31 Jul 2008 09:03:44 +0200

The CD15 MACSiBead™ Kit has been developed for the enrichment of lymphocytes from human whole blood by depletion of CD15+ cells, combined with the lysis of erythrocytes. The kit consists of CD15 MACSiBead Particles and Red Blood Cell Lysis Solution. CD15 MACSiBead Particles are superparamagnetic beads of approximately 3.5 µm in diameter, coupled to CD15 antibodies.
CD15 is expressed on neutrophils, eosinophils, and monoblastoid precursor cells of the myeloid lineage, but not on basophils and lymphocytes.

NEW: CD146 MicroBead Kit, human--for isolation of mesenchymal stromal cells as well as endothelial cells and pericytes

Thu, 31 Jul 2008 09:00:10 +0200

The CD146 MicroBead Kit was developed for the isolation of CD146+ cells. The kit consists of CD146 MicroBeads and FcR Blocking Reagent. The CD146 MicroBeads recognize the human CD146 antigen, also known as MUC18, MCAM, Mel-CAM, and S-Endo-1. CD146 is a transmembrane glycoprotein belonging to the immunoglobulin superfamily and contains five extracellular immunoglobulin (Ig)-like domains.
CD146 possesses a limited tissue expression distribution, including endothelial cells, smooth muscle cells, pericytes, follicular dendritic cells, melanoma cells, and a subpopulation of activated T lymphocytes. CD146+ cells isolated from umbilical cord, lipoaspirate, dental pulp, or endometrial tissue, are described to contain cells with clonogenic fibroblastic colony (CFU-F) activity and multilineage differentiation potential, which are often called mesenchymal or marrow stromal cells (MSCs).
CD146 functions as a Ca2+-independent cell adhesion molecule, which is involved in heterophilic cell-cell interactions, and may be involved in the extravasion and/or homing of activated T cells. CD146 expression in melanoma is also directly associated with tumor growth and metastasis.

NEW: Treg Detection Kits--complete kits for staining of mouse and human CD4+CD25+FoxP3+ regulatory T cells

Thu, 31 Jul 2008 08:56:14 +0200

The Treg Detection Kits, human, have been developed for the convenient detection of CD4+CD25+FoxP3+ regulatory T (Treg) cells by flow cytometry. Treg cells are a subset of T cells with the ability to suppress harmful immunological reactions to self and foreign antigens. Due to the lack of a specific marker for Treg cells, a combination of CD4, CD25, and FoxP3 is commonly used for their identification. The Treg Detection Kit includes all reagents necessary for cell surface staining of CD4 and CD25, and intracellular staining of FoxP3 with a special FoxP3 Staining Buffer Set. The kit also includes FcR Blocking Reagent and an optimized protocol.

NEW: Memory B Cell Isolation Kit

Tue, 17 Jun 2008 17:25:28 +0200

The Memory B Cell Isolation Kit has been developed for the isolation of memory B cells from human PBMCs. Memory B cells, defined as quiescent antigen-experienced B cells, are generated in response to T cell–dependent and T cell–independent antigens. They are able to react quickly to a recurrent antigenic challenge, thereby providing serological immune protection.
CD27, a member of the TNF receptor family, is expressed on most memory B cells. It allows their discrimination from naive B cells, which are CD27–.
Memory B cells are isolated by depletion of unwanted cells and subsequent positive selection with CD27 MicroBeads. Unwanted cells, for example, T cells, NK cells, monocytes, dendritic cells, granulocytes, platelets, and erythroid cells are depleted using a cocktail of biotinylated antibodies against CD2, CD14, CD16, CD36, CD43, and CD235a (glycophorin A), and Anti-Biotin MicroBeads. For evaluation of MACS® Separations, staining with CD19-APC and CD27-PE antibodies is recommended.

MICROGLIA: CD11b (Microglia) MicroBeads for the isolation of human microglial cells.

Thu, 24 Apr 2008 11:53:59 +0200

Microglia, often called brain macrophages, are the resident immune effector cells in the central nervous system (CNS). In addition, activated microglia serve as the major antigen-presenting cells in the CNS. They are morphologically, immunophenotypically, and functionally related to cells of the monocyte/macrophage lineage. The most characteristic feature of microglial cells is their rapid activation in response to injury, inflammation, neurodegeneration, infection, and brain tumors.
A paucity of microglial antigens has hindered microglial identification. However, CD11b has been widely used as marker for microglial identification and is also suitable for their immunomagnetic isolation from human brain tissue.
(see provided link for details)

GLIA: Anti-A2B5 MicroBeads and Anti-A2B5 pure antibody for the positive selection and analysis of glial-restricted precursor cells.

Thu, 24 Apr 2008 11:50:55 +0200

Anti-A2B5 MicroBeads have been developed for the isolation of A2B5+ cells. The Anti-A2B5 MicroBeads recognize the c-series ganglioside–specific antigen A2B5. Glycosphingolipids (GSLs) including gangliosides are considered to be useful cell-surface markers for monitoring neural stem and precursor cells and their progeny, and have been utilized as specific markers to define oligodendrocyte lineage cells during development.

STIMULATING: Peptide pools for efficient in vitro stimulation of human CMV- and AdV-specific T cells

Thu, 24 Apr 2008 11:48:44 +0200

The PepTivator - CMV IE-1 is a peptide pool that consists mainly of 15-mer peptides with 11–amino acid (aa) overlap, covering the complete sequence of the IE-1 protein of human cytomegalovirus (CMV).
The PepTivator – AdV5 Hexon is a peptide pool that consists mainly of 15-mer peptides with 11–amino acid overlap.
Adenoviruses (AdV) are non-enveloped, ubiquitous, lytic double-stranded DNA viruses. Up to now, 52 different human serotypes (1–52), divided into six species (A-F) are described.
The hexon protein is one of the structural proteins in the viral coat and is synthesized during late infection. It is the immunodominant T cell target among capsid proteins and contains multiple epitopes conserved among serotypes.

HUMAN TREGS: CD39 antibodies for characterization of human regulatory T cells and other CD39-expressing cells

Thu, 24 Apr 2008 11:44:28 +0200

The CD39 antibody specifically recognizes the membrane-bound human CD39 antigen, which is expressed on an effector/memory-like subset of FoxP3+ regulatory T cells1. CD39 is an ectonucleotidase and catalyzes the hydrolysis of extracellular nucleotides, for example, ATP. In concert with CD73, which is an ecto-5’-nucleotidase, this can lead to the production of adenosine.
High extracellular ATP concentrations indicate tissue injury and cell death and induce various pro-inflammatory responses in immune cells.
Through its enzymatic activity, CD39 can contribute to the suppressive function of regulatory T cells, for example, by eliminating extracellular ATP or by generating adenosine, which has suppressive effects on various immune cells.

ONE STEP ONLY: Cell analysis with just one pipetting step--the new MACS Control Antibody Cocktails

Thu, 24 Apr 2008 11:42:29 +0200

The MACS® Control (MC) Antibody Cocktails are designed for the easy and rapid evaluation of cell separation efficiency and purity by flow cytometry. After separation with MACS Technology, cells are simply incubated with the premixed cocktail and then analyzed. Minimal pipetting is necessary, making the procedure fast and easy for automation on the MACSQuant™ Analyzer. The cocktails can be used just as well in combination with other flow cytometers. Due to the addition of a CD45 antibody conjugated to VioBlue, the new fluorophore developed by Miltenyi Biotec for use with a violet laser, analysis can be easily restricted to leukocytes.

EXCITING: MACS Cytokines and Growth Factors--the wide selection for cell culture and bioassays

Thu, 24 Apr 2008 11:40:27 +0200

Miltenyi Biotec now offers a broad portfolio of cytokines, growth factors, and related proteins for human, mouse, and rat. The MACS® Cell Culture product line has been significantly expanded by including the valued cytokine selection of the former Strathmann Biotec AG, renowned for its extensive experience in manufacturing recombinant proteins with excellent purity and activity. These high-quality products are well-suited for various applications such as cell culture, differentiation studies, and biological assays. Selected products are available in a premium grade format for high, well-defined activity as well as in research grade quality.

MACS Antibodies--the bright side of life science

Thu, 24 Apr 2008 11:38:36 +0200

Miltenyi Biotec is constantly expanding its product range of antibodies and kits for cell analysis. Beside unique antibodies for stem cells and dendritic cells, we provide a large panel of fluorochrome-conjugated antibodies for murine, human, and non-human primate cells, as well as conveniently formulated kits for a variety of applications.
The MACS® Control Antibodies are optimized for the evaluation of cell fractions separated by MACS® Technology. The convenient protocol allows for staining during or after magnetic cell isolation using MACS® Technology.

Anti-IgM MicroBeads for the isolation or depletion of human IgM+ cells

Wed, 19 Mar 2008 11:51:07 +0100

Anti-IgM MicroBeads were developed for the enrichment or depletion of cells expressing IgM on their surface. The Anti-IgM MicroBeads recognize human IgM antibodies. They can also be used to isolate any cell type that is labeled with primary human IgM antibodies.

NEW: gentleMACS™ Dissociator—the gentle way of automated tissue dissociation

Fri, 01 Feb 2008 15:47:52 +0100

The gentleMACS™ Dissociator is a benchtop instrument for the automated dissociation of tissues. Two types of unique gentleMACS Tubes used with the instrument enable the time-saving and easy dissociation or homogenization of tissues in a closed system. C Tubes are used for the gentle preparation of single-cell suspensions from tissues, whereas M Tubes are used for the homogenization of tissues or cells. A single sample or two samples in parallel can be processed. The instrument offers optimized gentleMACS Programs for a variety of specific applications. The standardized tissue dissociation or homogenization procedures make for reliable and reproducible results.

STIMULATING: Treg Suppression Inspector, the ideal stimulation reagent for Treg suppression assays.

Thu, 31 Jan 2008 14:56:22 +0100

The Treg Suppression Inspector has been developed for the functional characterization of human CD4+CD25+ regulatory T (Treg) cells by in vitro suppression assays.
Treg cells are a subset of T cells that have the ability to suppress harmful immunological reactions to self or foreign antigens. This function of Treg cells can be analyzed by a so-called suppression assay. For this purpose, Treg cells and responder T cells are cocultured in different ratios in the presence of a polyclonal stimulus, in this case the Treg Suppression Inspector, while the proliferative response is measured.

NEW: CD137 MicroBead Kit

Thu, 31 Jan 2008 14:53:59 +0100

The CD137 MicroBead Kit is an indirect magnetic labeling system for the enrichment of human CD137+ cells. CD137+ cells are indirectly magnetically labeled with CD137-PE and Anti-PE MicroBeads. CD137-PE recognizes the CD137 (4-1BB) antigen, a 30 kDa glycoprotein of the tumor necrosis factor (TNF) receptor superfamily. CD137 is mainly expressed on activated CD4+ and CD8+ T cells, activated B cells, and on natural killer cells, but can also be found on resting monocytes and dendritic cells. As a costimulatory molecule CD137 is involved in the activation and survival of CD4+ or CD8+ T cells, and NK cells.

NEW: CD137-PE Antibody

Thu, 31 Jan 2008 14:54:13 +0100

For separation and analysis of activated antigen-specific T cells from human PBMCs. This antibody recognizes the CD137 (4-1BB) antigen, a 30 kDa glycoprotein of the tumor necrosis factor (TNF) receptor superfamily. CD137 is mainly expressed on activated CD4+ and CD8+ T cells, activated B cells, and natural killer cells, but can also be found on resting monocytes and dendritic cells.

NEW: CD57 MicroBeads

Thu, 31 Jan 2008 14:50:43 +0100

CD57 MicroBeads can be used for positive selection or depletion of CD57+ cells from human PBMCs, body fluids, or single-cell suspensions from tissue (e.g. lymphoid tissues). CD57 MicroBeads were developed for the separation of human CD57-expressing cells. The CD57 antigen, also known as HNK-1 or Leu-7, is an oligosaccharide antigenic moiety bound to surface proteins of certain cell types.

NEW: CD8+CD57+ T Cell Isolation Kit

Thu, 31 Jan 2008 14:49:16 +0100

The CD8+CD57+ T Cell Isolation Kit can be used for the isolation of CD8+CD57+ cells from PBMCs or other single-cell suspensions. The CD8+CD57+ T Cell Isolation Kit was developed for the isolation of CD57+ cytotoxic T cells. The separation of CD8+CD57+ T cells is performed in a two-step procedure. In a first step, CD8+ cells are magnetically labeled with CD8 MultiSort MicroBeads and isolated by positive selection. After the release of the magnetic particle, the CD8+CD57+ target cells are magnetically labeled with CD57 MicroBeads to be positively selected a second time.

NEW: CD57 Antibodies

Thu, 31 Jan 2008 14:47:39 +0100

For the identification and enumeration of CD57+ cells by flow cytometry and fluorescence microscopy. The antibody is suitable for use in MACS® Control applications.
CD57, also known as HNK-1 or Leu-7, is an antigenic oligosaccharide moiety detected on extracellular proteins of certain cell types. In blood, CD57 is found on 15–20% of mononuclear cells, including subsets of NK and T cells, though not on erythrocytes, monocytes, granulocytes, or platelets. Also, CD57 expression can be found on a variety of neural cell types.

NEW: CliniMACS® CD25 Reagent, CE-marked

Thu, 31 Jan 2008 14:44:34 +0100

CliniMACS® CD25 Reagent, CE-marked, for clinical-scale enrichment or depletion of CD25+ T cells on the CliniMACS® Plus Instrument.

One-Step Antibody Biotinylation Kit—small-scale biotinylation of any antibody for subsequent cell separation or analysis

Thu, 06 Dec 2007 12:30:16 +0100

The One-Step Antibody Biotinylation Kit has been developed for the biotinylation of monoclonal antibodies for use in magnetic cell separation (with MACS® Technology), as well as in fluorescent cell analysis.

NEW: Naive CD8+ T Cell Isolation Kit for human PBMCs.

Thu, 06 Dec 2007 12:27:28 +0100

The Naive CD8+ T Cell Isolation Kit was developed for the isolation of human naive CD8+ T cells from PBMCs. Naive CD8+ T cells isolated with the Naive CD8+ T Cell Isolation Kit can be used for functional T cell studies, e.g., studies on signal transduction or cytokine expression after activation of the naive CD8+ T cells.

PROFILING: More miRXplore™ Microarray Kits

Thu, 06 Dec 2007 12:23:42 +0100

miRXplore™ Microarray Kits now available for microRNA expression profiling covering human, mouse, rat and viral sequences (miRBase v.10).

STIMULATING: PepTivator - CMV pp65

Thu, 06 Dec 2007 12:20:54 +0100

PepTivator - CMV pp65 is a peptide pool that consists mainly of 15-mer peptides with 11 amino acid (aa) overlap, covering the complete sequence of the pp65 protein of human cytomegalovirus (CMV).

PepTivator - CMV pp65 has been especially developed for efficient in vitro stimulation of CMV pp65-specific CD4+ and CD8+ T cells, as peptides of 15 aa length with 11 aa overlap represent an optimized solution for stimulating both CD4+ and CD8+ T cells in various applications.

MORE NEWS: Miltenyi Biotec eNewsletter, November 2007

Wed, 14 Nov 2007 12:21:56 +0100

The November issue of the Miltenyi Biotec eNewsletter is now available – with these topics:

Miltenyi Biotec introduces the first PepTivator: a high-quality peptide pool for antigen-specific immunostimulation of T cells.

Free copy of Thomas Tuschl's miRNA paper for eNews readers
Thomas Tuschl, world-leading miRNA scientist and researcher, uses Miltenyi Biotec’s miRXplore™ Microarrays. miRXplore™ Microarrays have successfully been used in a recently published study in Cell on building up the most comprehensive microRNA expression atlas available up to date.

MACS® Antibodies—your perfect solution for flow cytometry!
Looking for new antibodies? Choose from our portfolio of high-quality fluorochrome conjugates!

Stimulate • Activate • Expand: new tools for functional assays
Have a look at our expanding range of new antibodies perfectly suited for a multitude of functional assays! All products are endotoxin-tested and free of azide and stabilizer.

Autoimmunity research: multiple sclerosis
Would you like to isolate regulatory T cells for your MS research? Do you need isolated leukocytes for adoptive transfer experiments in MS mouse models?

We are the dendritic cell experts!
Miltenyi Biotec has developed a wide range of innovative and reliable products for isolation and analysis of mouse and human DCs and DC subsets.

NEW! Cell type–specific total RNA from distinct human hematopoietic cell populations

To subscribe, please use the link provided with this RSS feed or go directly to http://www.miltenyibiotec.com/en/newsletter_subscribe.aspx.

STATE-OF-THE-ART: miRXplore™ Microarray Services

Mon, 05 Nov 2007 13:21:33 +0100

Profit from the cost-effective, trusted, and fast miRNA profiling services of Miltenyi Biotec, avaliable in two types:
i) miRXplore™ Microarray Service
a standard service using sample and control both sent in by the researcher,
ii) miRXplore™ Microarray Universal Reference Service
using a synthetic miRNA pool as a reference. This option can be used if multiple samples have to be compared and sample material has to be saved.

MORE NEWS: Miltenyi Biotec eNewsletter, October 2007

Fri, 19 Oct 2007 11:08:48 +0200

In the October edition of the Miltenyi Biotec eNewsletter, you can find out about new products for isolation of stem cells or Tregs, diabetes research, and miRNA expression profiling. Just follow the links in each article to find out more.

Contents of this issue:

* Special offer: Buy 5 products and pay for 4!
* NEW! microRNA expression profiling with miRXplore™ Microarray Kits and Services
* New solutions for mouse stem cell isolation
* Choices for isolation of human regulatory T cells
* Recombinant IFN-α, mouse
* Autoimmunity - Diabetes research
* eShop: Latest pricing and online ordering
* Meetings and congresses

To subscribe, please use the link provided with this RSS feed or go directly to http://www.miltenyibiotec.com/en/newsletter_subscribe.aspx.

READY-TO-USE: Cell type-specific total RNA for gene expression profiling.

Fri, 19 Oct 2007 10:27:37 +0200

Reliable cell-type specific RNA—from cells separated with MACS® Technology for optimal purity and gentle processing. The isolation of highly pure total RNA samples from distinct hematopoietic cell populations is laborious and costly to achieve. This is especially true for rare and immunologically relevant cell populations such as regulatory T cells (CD4+ CD25+). Each cell-type specific total RNA is a high-quality RNA derived from various hematopoietic cell populations, ready-to-use for gene expression profiling experiments, gene cloning, or as a reference for further analyses.

RECOMBINANT: IFN-alpha, mouse

Mon, 17 Sep 2007 18:04:33 +0200

Recombinant IFN-α, mouse, can be used for studies of in vitro and in vivo effects of IFN-α in mouse models, e.g., for the in vitro investigation of antitumor effects (antiproliferative capacities) of IFN-α on tumor cell lines or for stimulation of IFN-α-dependent cell lines.
In vivo, recombinant mouse IFN-α can be utilized in studies using transgenic mice harboring the Cre-recombinase transgene driven by the interferon-inducible Mx promoter (Mx-Cre mice).
The recombinant IFN-α mouse was expressed in HEK293 cells and has been purified and analyzed for its activity.

DEPLETION INCLUDED: CD4+CD25+CD127dim/– Regulatory T Cell Isolation Kit, human

Mon, 17 Sep 2007 18:02:02 +0200

The CD4+CD25+CD127dim/- Regulatory T Cell Isolation Kit has been developed for the isolation of CD4+CD25+CD127dim/- cells from PBMCs. The kit contains a cocktail of biotinylated antibodies and Anti-Biotin MicroBeads for the depletion of non-CD4+ and CD127high cells, and CD25 MicroBeads for the subsequent positive selection of CD4+CD25+CD127dim/- cells.

CLINICAL SCALE: CliniMACS® CD4 Reagent, CE-marked, for enrichment or depletion of CD4+ T cells on the CliniMACS® Plus Instrument

Mon, 17 Sep 2007 17:59:10 +0200

CD4 is an accessory molecule in the recognition of foreign antigens in association with MHC class II antigens by T cells. T helper cells and to a lower degree monocytes and dendritic cells express CD4. The CliniMACS® CD4 Reagent and MicroBeads consist of superparamagnetic iron dextran particles directly conjugated to CD4 antibodies. One vial contains 7.5 mL sterile, non-pyrogenic solution suitable for magnetic labeling of up to 12×10e9 CD4+ cells from up to 40×10e9 total cells.

CHILLING: MACS Cooling Tube Racks

Mon, 17 Sep 2007 17:54:28 +0200

MACS® Cooling Tube Racks are valuable tools for any procedure where cooling of sample tubes is desired. Cooling of cell samples helps to sustain cell viability during cell analysis or cell separation. The tube racks are pre-cooled in the refrigerator (2–8 °C). The special coolant contained in the tube racks switches from fluid to solid and maintains a low temperature until the coolant is completely melted.

HUMAN NEUTROPHILS: Separation and analysis using CD66abce MicroBead Kit and Antibodies

Mon, 17 Sep 2007 17:52:54 +0200

CD66abce MicroBeads are used for the isolation or depletion of human neutrophils from lysed human peripheral blood. Neutrophils play a prominent role in the innate immune response through their phagocytic activity. They are abundant in peripheral blood but almost absent in healthy tissue. Upon infection, large numbers of neutrophils are recruited to the sites of inflammation.
The CD66abce Antibody is suitable for the identification and enumeration of CD66abce+ human cells by flow cytometry or fluorescence microscopy.

INFORMATIVE: The latest issue of MACS&more is available for free download.

Thu, 12 Jul 2007 14:43:44 +0200

MACS&more presents valuable information on the isolation and gene expression profiling of natural killer cell subsets, presents data on CD271 (LNGFR)+ cells, provides experimental data of adapting manual hybridization protocols to the a-Hyb™ Hybridization Station, and introduces a load of new products, including the autoMACS® Pro Separator, Isolation kits for NK cell subsets, the NKT Cell Isolation Kit, the CD154 MicroBead Kit, the µMACS™ One-step cDNA Labeling Kit, and last, but not least, the PrepProtect™ Stabilization Buffer, awarded with the Frost&Sullivan "Product Innovation of the Year Award 2006".

GENTLE AND EFFICIENT: Yield viable neuronal progenitor cells of high purity within just hours with the Neural Tissue Dissociation Kit (Trypsin) and Anti-PSA-NCAM MicroBeads.

Thu, 12 Jul 2007 14:31:53 +0200

The Neural Tissue Dissociation Kit (Trypsin) has been developed for the gentle, but efficient, generation of single-cell suspensions from neural tissues. In less than an hour, the gentle two-step enzymatic dissociation procedure efficiently yields high numbers of viable cells. Neural tissues from embryonic, postnatal and adult murine tissue sections or whole brain preparations can be processed. Dissociated cells are immediately ready for further in vitro or in vivo applications, including MACS® Cell Separations.
Anti-PSA-NCAM MicroBeads have been developed for the isolation of PSA-NCAM+ cells. The anti-PSA-NCAM antibody recognizes polysialic acid (PSA) which, in vertebrates, is linked to the extracellular domain of the neural cell adhesion molecule (NCAM, CD56). PSA-NCAM, the highly polysialated form of NCAM, is predominantly expressed in embryonic and neonatal neural tissue. In adult mammalian brain PSA-NCAM expression is restricted mainly to areas that retain neurogenic potential, such as the subventricular zone (SVZ) and the dentate gyrus of the hippocampus.

NEW: Anti-IFN-α-antibodies for the intracellular detection of IFN-α in human cells

Thu, 12 Jul 2007 14:25:42 +0200

The antibody was developed for intracellular staining of IFN-α in fixed and permeabilized cells for flow cytometric analysis. Cells can be stained in suspension or by solid-phase intracellular staining technology in combination with MACS® Cell Separation.
Type I interferons (IFNs), including IFN-α, are a family of cytokines that exert pleiotropic functions in the immune system. Firstly described for its potent antiviral activity, IFN-α exerts a variety of immunmodulatory effects, including positive and negative regulation of its own production, regulation of IL-12 and IFN-γ production, promotion of natural killer (NK) cell cytotoxicity, activation and maturation of dendritic cells. and the promotion of B cell development towards plasma cells. IFN-α is expressed predominantly by CD123+ CD11c– CD303 (BDCA2)+ plasmacytoid dendritic cells (pDCs).

NEW: Enrichment of human SLAN (M-DC8)+ monocytes

Thu, 12 Jul 2007 14:22:56 +0200

The Slan (M-DC8) Monocyte Isolation Kit is designed for the magnetic separation of human Slan (M-DC8)+ cells. The kit contains CD3 MicroBeads for efficient depletion of contaminating T cells, FcR Blocking Reagent and Anti-Slan (M-DC8) MicroBeads.

NEW: Detection of human SLAN (M-DC8)+ monocytes

Thu, 12 Jul 2007 14:21:49 +0200

Slan (6-Sulfo LacNAc) is a carbohydrate modification of P-selectin glycoprotein ligand-1 (PSGL-1) characteristically expressed on a new subset of peripheral blood mononuclear cells (PBMCs) with features closely related to CD16+CD14low monocytes.

NEW: Products for staining of CD28+ cells

Thu, 12 Jul 2007 14:18:33 +0200

The CD28 antibody can be used to identify and enumerate cells by flow cytometry or fluorescence microscopy.
CD28 is a type I transmembrane protein, highly expressed on CD3+ thymocytes, most peripheral thymocytes and plasma cells. Binding of CD28 to its ligands CD80 or CD86 co-stimulates T cell effector function and T cell dependent antibody production in vitro and in vivo.

NEW: Products for separating human CD28+ cells

Thu, 12 Jul 2007 14:16:26 +0200

The CD28 MicroBead Kit is an indirect magnetic labeling system for the enrichment of CD28+ cells from human PBMCs. CD28+ cells are indirectly magnetically labeled with CD28-PE antibody and Anti-PE MicroBeads.

HIGHLIGHT: Cell paper with active participation of Miltenyi Biotec scientists.

Tue, 10 Jul 2007 12:59:22 +0200

A research group headed by Tom Tuschl, Rockefeller University New York, has published a major miRNA research article in the journal Cell — also based on the active participation of Miltenyi Biotec scientists using the upcoming miRNA miRXplore™ Microarrays: Landgraf et al. (2007) Cell 129, 1401–1414.

EVEN BETTER: CD4+CD62L+ T Cell Isolation Kit II for the isolation of naive mouse CD4+ T cells – new version!

Fri, 29 Jun 2007 13:20:36 +0200

The CD4+CD62L+ T Cell Isolation Kit II has been developed for the isolation of CD4+CD62L+ T helper cells from spleen and lymph nodes.
CD62L (L-selectin) is highly expressed on naive T cells and down-regulated upon activation. It is also expressed on a small subset of memory T helper cells, the central memory T cells, which can be distinguished from naive T helper cells by their high expression of CD44. Furthermore, CD62L is expressed on most thymocytes, naive CD8+ T cells, B cells, dendritic cells, macrophages, NK cells, neutrophils, eosinophils, regulatory T cells, and TCRγ/δ+ T cells. For isolation of CD4+CD62L+ T helper cells, the non-T helper cells as well as regulatory T cells and γ/δ T cells are depleted by indirect magnetic labeling using a cocktail of lineage-specific biotin-conjugated antibodies against CD8a (Ly-2), CD45R (B220), CD49b (DX5), CD11b (Mac-1), and Ter-119, as well as antibodies against CD25 and TCRγ/δ in combination with Anti-Biotin MicroBeads. Subsequently, CD4+CD62L+ T cells are positively selected from the enriched CD4+ T helper cell fraction with CD62L (L-selectin) MicroBeads.

NEW: Products for the enrichment of activated mouse CD154+ T helper cells.

Fri, 29 Jun 2007 13:18:51 +0200

The CD154 Enrichment and Detection Kit is designed for the easy identification and enrichment of antigen-specific CD4+ T cells. Detection and stimulation of the cells are conveniently performed in one step.
CD154, also known as CD40L, gp39, T-BAM, and TRAP is transiently up-regulated on activated CD4+ T cells and plays an important role as a costimulatory molecule in the interaction between T cells and antigen-presenting cells through ligation of CD40. Due to its transient expression within hours of activation, CD154 can be used as a marker for activated antigen-specific CD4+ T cells.
Using the CD154 Enrichment and Detection Kit, antigen-specific CD4+ T cells are indirectly magnetically labeled with a CD154 Detection Cocktail (PE) and anti-PE MicroBeads.
To optimize the detection of CD154 on stimulated antigen-specific CD4+ T cells, cells are incubated simultaneously with the desired antigen in vitro to induce the expression of CD154 and with the CD154 Detection Cocktail. Besides a PE-conjugated CD154 antibody, the CD154 Detection Cocktail contains a CD40 blocking antibody to prevent the down-regulation of CD154 surface expression, and a CD28 antibody to provide a costimulatory signal.
After stimulation, cells are magnetically labeled with anti-PE MicroBeads and separated on a MACS Column.

NEW: Products for the detection of activated mouse CD154+ T helper cells.

Fri, 29 Jun 2007 13:17:09 +0200

The CD154 Detection Cocktail is designed for the easy identification of activated antigen-specific CD4+ T cells. Cells are stimulated with the desired antigen in vitro to induce the expression of CD154 and are simultaneously incubated with the CD154 Detection Cocktail.

USEFUL COMPANION: A special protocol for the isolation of mouse CD105+Sca-1+ LTR-HSCs is now available.

Fri, 29 Jun 2007 13:15:20 +0200

This special protocol describes the isolation of CD105+ Sca-1+ HSCs from mouse bone marrow using a two-step magnetic purification procedure. After initial selection for CD105 expression, purified cells are magnetically labeled for a second time to select for Sca-1 expression. After the second step, the resulting cell population will be CD105+ Sca-1+ LTR-HSCs.
Sequential magnetic purification of the target cells is facilitated by using the CD105 MultiSort Kit (PE), mouse (# 130-092-924) in the first dimension, which includes a MicroBead removal step before cells are labeled for a second time with the Anti-Sca-1 MicroBead Kit (FITC), mouse (# 130-092-529).
The complete protocol is available for download now.

NEW: CD105 MultiSort Kit (PE), mouse, for the multiparameter isolation of CD105+ HSCs from mouse bone marrow.

Fri, 29 Jun 2007 13:10:30 +0200

The CD105 MultiSort Kit (PE), mouse is an indirect magnetic labeling system designed for the isolation of distinct CD105+ subsets from mouse blood, bone marrow, or cell cultures. The CD105+ cells are isolated using CD105 MultiSort MicroBeads, after which magnetic particles are enzymatically released. The CD105+ cells can then be magnetically labeled and sorted for a second antigen. The CD105 MultiSort Kit (PE), mouse is a complete system for sequential positive selections. It contains CD105-PE, mouse, Anti-PE MultiSort MicroBeads, the MultiSort Release Reagent, and the MultiSort Stop Reagent.

Positive II: CD271-PE–based positive selection of human marrow stromal cells (MSCs) with the MSC Research Tool Box – CD271 (LNGFR).

Fri, 29 Jun 2007 13:09:07 +0200

The MSC Research Tool Box – CD271 (LNGFR) has been developed for the standardized and optimized enrichment and expansion of human marrow stromal cells (MSCs) from bone marrow aspirates. The tool box includes all necessary reagents for fluorescent labeling (CD271 (LNGFR)-PE or -APC) and indirect magnetic labeling (Anti‑PE or -APC MicroBeads) of CD271 (LNGFR)+ cells, as well as FcR Blocking Reagent to block unspecific labeling via Fc receptors. Furthermore, the MSC Research Tool Box – CD271 (LNGFR) contains NH Expansion Medium, a specialized medium for the expansion of MSCs, and CytoMix – CD271 (LNGFR), a cytokine cocktail designed specifically for the optimal and reproducible expansion of CD271 (LNGFR)+ MSCs.

Positive I: Selection of human marrow stromal cells (MSCs) with the CD271 MicroBead Kit.

Fri, 29 Jun 2007 13:07:52 +0200

The CD271 (LNGFR) MicroBead Kit is an indirect labeling system for the immunomagnetic isolation of CD271 (LNGFR)+ cells. The kit includes all necessary reagents for fluorescent labeling (CD271 (LNGFR)-PE or -APC) and indirect magnetic labeling (Anti-PE or -APC MicroBeads) of CD271 (LNGFR)+ cells, as well as FcR Blocking Reagent for the prevention of unspecific labeling via Fc receptors.

NEW: Whole Blood CD138 MicroBeads

Tue, 29 May 2007 14:41:09 +0200

Whole Blood CD138 MicroBeads are suitable for the isolation of plasma cells directly from whole blood or bone marrow with the autoMACS™ Pro Separator and the autoMACS™ Separator.

NEW: Plasmacytoid Dendritic Cell Isolation Kit II, mouse

Tue, 29 May 2007 14:38:28 +0200

The Plasmacytoid Dendritic Cell Isolation Kit II is designed for the isolation of untouched plasmacytoid dendritic cells (PDCs) from single-cell suspensions of lymphoid organs.

GET THE TREGS: New FoxP3 antibodies

Tue, 29 May 2007 14:37:01 +0200

FoxP3, also known as FORKHEAD BOX P3, SCURFIN, and JM2, is a member of the forkhead/winged-helix family of transcriptional regulators. It is expressed predominantly in Tregs and is a major marker and functional regulator of T reg cell development and function.Mutations in the FoxP3 gene have been shown to be linked to the autoimmune manifestations observed in the Scurfy mouse and in humans with immune dysregulation, polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) disease. Studies in the mouse showed that FoxP3-deficient animals lack T reg cells, whereas overexpression of the FoxP3 protein leads to profound immune suppression1. The Anti-FoxP3 antibody, clone 3G3, detects human as well as mouse FoxP3 2. Read more on our website.

NEW: CD209 (DC-SIGN) antibody

Thu, 26 Apr 2007 17:27:16 +0200

Antibody CD209 (DC-SIGN) can be used for the identification and enumeration of CD209 (DC-SIGN)+ human cells by flow cytometry or fluorescence microscopy.

NEW: CD209 (DC-SIGN) MicroBead Kit

Thu, 26 Apr 2007 17:26:15 +0200

The CD209 (DC-SIGN) MicroBead Kit is designed for the magnetic separation of human CD209+ cells. The CD209 (DC-SIGN) MicroBead Kit can be used for positive selection or depletion of cells expressing the human CD209 (DC-SIGN) antigen.

NEW: CliniMACS Biotinylation Kit 500 for Research Use

Thu, 26 Apr 2007 17:21:58 +0200

Biotinylate your own antibody for subsequent CliniMACS Anti-Biotin Separation. For research use only, not for use in humans.
The CliniMACS® Biotinylation Kit 500 for Research Use has been developed for the biotinylation of monoclonal antibodies for further magnetic cell selection from leukapheresis products for research applications using the CliniMACS Plus Instrument. One CliniMACS Biotinylation Kit 500 for Research Use is sufficient for biotinylating 500 μg monoclonal antibody.
For availability in your country, please contact your local representative.

NEW: IL-12 Cytokine Secretion Assays

Thu, 26 Apr 2007 17:19:43 +0200

IL-12 Cytokine Secretion Assays, human, for enrichment and detection of IL-12–secreting leukocytes, are now available from Miltenyi Biotec.
Interleukin-12 (IL-12) is a 70–75 kDa heterodimeric cytokine composed of a 40 kDa heavy chain and a 35 kDa light chain. It is mainly produced by monocytes and macrophages, as well as dendritic cells primarily in response to stimulation with bacterial or viral antigens. IL-12 displays various effects on both NK and T cells, playing an essential role in the interaction between innate and adaptive immunity.
The IL-12 Secretion Assay is designed to detect and enrich cells secreting the p70 subunit of human IL-12.

JUST SENT: The latest MACS eNewsletter

Thu, 26 Apr 2007 17:04:46 +0200

The latest MACS eNewsletter has just been sent out via e-mail. Should you also wish to receive useful product information, take a moment and subscribe to the MACS eNewsletter by Miltenyi Biotec.

ELECTRONICALLY: Subscribe to the latest product information via e-mail!

Thu, 12 Apr 2007 17:09:15 +0200

Details on our "Tools for tumor immunology research" has just been sent out via e-mail. Should you also wish to receive useful product information, take a moment and subscribe to the MACS eNewsletter by Miltenyi Biotec.

INDISPENSABLE: Tools for hematopoietic stem cell research

Thu, 08 Mar 2007 16:54:24 +0100

Hematopoietic stem and progenitor cell (HSC/HPC) research has contributed a great deal to the understanding and harnessing of hematopoieisis for use within therapeutic research applications.
Miltenyi Biotec provides integrated product solutions for cutting-edge human hematopoietic stem and progenitor cell research. All products for human HSCs/HPCs research have been optimized for direct use with one another, meaning that isolation, cultivation, and analysis of HSCs can be performed in a standardized, seamless process.

NEW: Intracellular Cytokine Detection Kits

Wed, 07 Mar 2007 12:13:15 +0100

Miltenyi Biotecs Intracellular Cytokine Detection Kits are designed for rapid and reliable flow cytometric detection of cytokine production in activated CD4+ T cells. As a specific and functional marker of T cell activation, CD154 is used. CD154 is transiently up-regulated on activated CD4+ T cells within hours after activation. By combining the detection of cytokine secreting cells with staining of CD154 and CD4, antigen specific TH cell responses can be directly identified.
The Kits contain all reagents necessary for the detection of cytokine-producing activated CD4+ T cells from whole blood or PBMCs by intracellular staining. In addition, reagents are included for the fixation and permeabilization of cells prior to immunofluorescent staining, as well as for the in vitro stimulation of T cells as a positive control.

NEW: MultiMACS™ Separator for automated 96-well isolation of proteins, mRNA, and viral particles

Tue, 13 Feb 2007 17:55:02 +0100

High throughput and performance in molecule purification—the MultiMACS Separator allows the parallel processing of multiple samples with MACS® Technology.
Manual or robotic molecule isolation—the convenient and fast μMACS™ Procedures are performed by manual pipetting with software guidance or fully automated in a liquid handling robot.
Parallel processing of up to 96 samples—the flexible format allows performance of 8 to 96 reactions in parallel using Multi-8 Columns or Multi-96 Columns.
The MultiMACS Separator is a semi-automated benchtop magnet for multiple magnetic sample applications in molecular biology and protein biochemistry. Highly pure molecules, such as mRNA, specific nucleic acid sequences, or proteins, as well as molecular complexes, can be isolated with MACS Technology. Further applications include isolation of molecular complexes for protein interaction studies, library separation using display technologies, or purification of live, infectious virus particles.
Well-proven MACS Technology automated with the MultiMACS Separator reduces time, handling steps and pipette tips while increasing purity and sensitivity.

AWARD: Frost&Sullivan Product Innovation of the Year

Fri, 05 Jan 2007 16:20:03 +0100

Frost & Sullivan Product Innovation of the Year Award was presented to Miltenyi Biotec for the PrepProtect Stabilization Buffer.

NEW: Online ordering at Miltenyi Biotec

Fri, 05 Jan 2007 16:18:07 +0100

Order online by using our new "Customer Login" option! We hope you will enjoy the benefits of creating your own account, including a favorites list, in which you can save all the products you are currently using. Not only you, but also your colleagues can use this list in order for you to minimize purchasing time and tedious hand-written order requests.

ONE DAY ONLY: T7 amplification from magnetic RNA isolation to in-column amplification with μMACS™ One-step T7 Template Kit.

Fri, 05 Jan 2007 16:13:11 +0100

Linear T7 amplification with paramagnetic beads – The revolutionary process simplifies T7 cDNA template synthesis from limited biological samples, such as biopsies or low numbers of cells.

WALK-AWAY CELL SORTING: The new autoMACS™ Pro Separator

Fri, 05 Jan 2007 16:13:23 +0100

The autoMACS™ Pro Separator combines all the benefits of the autoMACS™ Separator with novel features such as the processing of up to six samples and sensor-controlled operation. Thus, hands-on time during cell separation is minimized. Color-coded illumination of buffer bottles allows monitoring of the instrument status. The instrument is operated via touchscreen and intuitive screen menus. Three different tube racks allow the use of sample volumes from 0.2 mL up to 50 mL.

NEW: NK Cell Isolation Kit, Human

Fri, 05 Jan 2007 16:13:40 +0100

The NK Cell Isolation Kit, Human, replaces the current NK Cell Isolation Kit, II (130-091-152). The new kit achieves a more consistent CD3+ cell depletion, even when high numbers of contaminating NKT cells are present in the sample.

NEW: Anti-His-tag antibodies

Fri, 05 Jan 2007 16:13:48 +0100

HRP-, FITC-, PE-, and Biotin- conjugated Anti-His-tag antibodies for the detection of poly-histidine-tagged proteins in Western Blot and ELISA analyses, flow cytometry or fluorescence microscopy.

NEW: Basophil Isolation Kit II, human

Fri, 05 Jan 2007 16:13:56 +0100

As of September 15, 2006, this kit for the isolation of untouched basophils substitutes the Basophil Isolation Kit, order no. 130-053-401.

NEW: CD154 MicroBead Kit, human

Fri, 05 Jan 2007 16:14:02 +0100

New kit for the isolation of antigen-specific CD4+ T cells based on the expression of CD154 (CD40L)

CATCH THE KILLERS: Isolate NK cell subsets!

Fri, 05 Jan 2007 16:14:09 +0100

New MACS® Cell Separation Kits for specific isolation of NK cell subsets: CD56bright NK cells, CD56dim NK cells, and CD8+/– NK cells.

ANOTHER PROFILER: PIQOR™ Ubiquitin-PS Microarray

Fri, 05 Jan 2007 16:14:15 +0100

Investigate the ubiquitin-proteasome system with PIQOR™ Ubiquitin-PS Microarrays.

NEGATIVE BUT DOMINANT: Double-negative T Cell Isolation Kit

Fri, 05 Jan 2007 16:14:23 +0100

New kit for easy isolation of human CD4-CD8-CD3+TCRα/β+ T cells

EXPANDED: More antibodies for intracellular cytokine staining.

Fri, 05 Jan 2007 16:14:29 +0100

Now available: Anti-IFN-γ, mouse; Anti-IL-2, mouse; Anti-TNF-α, mouse

NEW: Neural Tissue Dissociation Kit

Fri, 05 Jan 2007 16:14:35 +0100

Neural Tissue Dissociation Kit for gentle and efficient dissociation of murine neural tissues

FOR ANALYSIS: New NK-specific antibodies!

Fri, 05 Jan 2007 16:14:40 +0100

New NK cell–specific antibodies: CD158e (KIR3DL1), human; CD226 (DNAM-1), human; CD335 (NKp46), human; CD336 (NKp44), human; CD337 (NKp30), human/rhesus

NEW: Anti-Prominin-1 MicroBeads and Antibodies

Fri, 05 Jan 2007 16:14:52 +0100

Anti-Prominin-1 MicroBeads and Antibodies for the enrichment and detection of Prominin-1 positive cells; e.g., from neural, kidney, and hematopoietic tissue.

INNOVATIVE: In-column cDNA labeling for microarrays

Fri, 05 Jan 2007 16:14:59 +0100

The new μMACS™ One-step cDNA Kit combines the efficient direct magnetic isolation of mRNA with a revolutionary in-column cDNA synthesis.

INDEPENDENT: MACSmix™ Tube Rotator

Fri, 05 Jan 2007 16:15:06 +0100

Mix wherever you are: The versatile MACSmix Tube Rotator running on rechargeable batteries and operating independently from a permanent power supply.

EXCITING: Positive selection of marrow stromal cells

Fri, 05 Jan 2007 16:15:13 +0100

CD271 (LNGFR) MicroBead Kit, human, for the enrichment of marrow stromal cells (MSCs) from bone marrow

ACTIVE CIRCULATION: The new a-Hyb™ Hybridization Station

Tue, 02 May 2006 16:40:39 +0200

Fast and reproducible results due to active circulation

NEW: SuperAmp Service

Fri, 05 Jan 2007 16:15:33 +0100

RNA amplification service for single-cell gene expression profiling

COMING SOON: The MACS eNewsletter

Fri, 28 Apr 2006 16:02:42 +0200

Subscribe now to receive up-to-date information about Miltenyi Biotec products and services.

NEW: PrepProtect

Fri, 05 Jan 2007 16:15:43 +0100

Stabilize fresh or frozen samples for reliable gene expression analysis.

COURSE: "Genome, transcriptome, and miRNome analysis of single cells"

Fri, 28 Apr 2006 12:32:19 +0200

EMBO practical course organized by Miltenyi Biotec.

WWW: New banner ads launched!

Fri, 28 Apr 2006 12:32:34 +0200

Now available: Direct links to BSA Stock Solution, Pre-Separation Filters, the MACSmix Tube Rotator, and the a-Hyb Hybridization Station.